Loss of integrin linked kinase from mouse hepatocytes in vitro and in vivo results in apoptosis and hepatitis.

Abstract

UNLABELLED:

Extracellular matrix (ECM) is fundamental for the survival of cells within a tissue. Loss of contact with the surrounding ECM often causes altered cell differentiation or cell death. Hepatocytes cultured without matrix lose patterns of hepatocyte-specific gene expression and characteristic cellular micro-architecture. However, differentiation is restored after the addition of hydrated matrix preparations to dedifferentiated hepatocytes. Integrin-linked kinase (ILK) is an important component of cell-ECM adhesions transmitting integrin signaling to the interior of the cell. ILK has been implicated in many fundamental cellular processes such as differentiation, proliferation, and survival. In this study, we investigated the role of ILK in mouse hepatocytes in vitro as well as in vivo. Depletion of ILK from primary mouse hepatocytes resulted in enhanced apoptosis. This was accompanied by increased caspase 3 activity and a significant decrease in expression of PINCH and alpha-parvin, which, along with ILK, form a stable well-characterized ternary complex at cell-ECM adhesions. The induction of apoptosis caused by ILK depletion could be substantially reversed by simultaneous overexpression of ILK, indicating that apoptosis is indeed a consequence of ILK removal. These results were further corroborated via in vivo data showing that adenoviral delivery of Cre-recombinase in ILK-floxed animals by tail vein injection resulted in acute hepatitis, with a variety of pathological findings including inflammation, fatty change, and apoptosis, abnormal mitoses, hydropic degeneration, and necrosis.

CONCLUSION:

Our results demonstrate the importance of ILK and integrin signaling for the survival of hepatocytes and the maintenance of normal liver function.

• Authors: Vasiliki Gkretsi, Wendy M Mars, William C Bowen, Lindsay Barua, Yu Yang, Lida Guo, René St-Arnaud, Shoukat Dedhar, Chuanyue Wu, George K Michalopoulos
• Journal: Hepatology (Baltimore, Md.) (Hepatology) Vol. 45 Issue 4 Pg. 1025-34 (Apr 2007) ISSN: 0270-9139 [Print] United States
• PMID: 17385211 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Adaptor Proteins, Signal Transducing
• DNA-Binding Proteins
• Integrin beta1
• LIM Domain Proteins
• Lims1 protein, mouse
• Membrane Proteins
• Microfilament Proteins
• Parva protein, mouse
• integrin-linked kinase
• Protein Serine-Threonine Kinases
• Proto-Oncogene Proteins c-akt
• Cre recombinase
• Integrases

Topics

• Adaptor Proteins, Signal Transducing
• Adenoviridae (genetics)
• Animals
• Apoptosis (physiology)
• Cells, Cultured
• DNA-Binding Proteins (metabolism)
• Extracellular Matrix (metabolism)
• Gene Expression
• Hepatitis (pathology, physiopathology)
• Hepatocytes (metabolism, physiology)
• Integrases (genetics)
• Integrin beta1 (metabolism)
• LIM Domain Proteins
• Liver (pathology)
• Membrane Proteins
• Mice
• Mice, Knockout
• Microfilament Proteins (metabolism)
• Phosphorylation
• Protein Serine-Threonine Kinases (genetics, metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• Transfection