Neutrophils of healthy subjects with a history of reactive arthritis show enhanced responsiveness, as defined by CD11b expression in adherent and non-adherent whole blood cultures.

Abstract

OBJECTIVES:

To study innate immune responsiveness of HLA-B27 positive subjects recovered from Yersinia-triggered reactive arthritis (B27 + ReA+).

METHODS:

Whole blood samples from 15 B27 + ReA+, 15 B27 + ReA- and 15 B27 - ReA- subjects were heparinized, aliquoted and (i) kept at 0 degree C to preserve constitutive cell surface marker status, or (ii) cultured with or without bacterial lipopolysaccharide (LPS) supplement, in adherent and non-adherent conditions at 37 degrees C for 4 h. Neutrophil surface expression of CD11b, CD14 and CD16 was quantified flow cytometrically, and compared between the subject groups using Jonckheere-Terpstra test.

RESULTS:

The B27 + ReA+ group showed significantly higher CD11b levels than the B27 - ReA- group on non-adherent neutrophils cultured with LPS as 100 pg/ml (P = 0.027), 10 ng/ml (P = 0.048) or 1 microg/ml (P = 0.024), or on adherent neutrophils without LPS supplement (P = 0.040). CD14 and CD16 expression on cultured neutrophils and constitutive expression of all three markers were comparable between the groups.

CONCLUSIONS:

Enhanced neutrophil reactivity observed may exacerbate innate immune inflammation in HLA-B27 positive ReA patients.

• Authors: K Kuuliala, A Orpana, M Leirisalo-Repo, H Repo
• Journal: Rheumatology (Oxford, England) (Rheumatology (Oxford)) Vol. 46 Issue 6 Pg. 934-7 (Jun 2007) ISSN: 1462-0324 [Print] England
• PMID: 17384172 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD11b Antigen
• HLA-B27 Antigen
• Lipopolysaccharide Receptors
• Lipopolysaccharides
• PHB2 protein, human
• Prohibitins
• Receptors, IgG
• Tumor Necrosis Factor-alpha

Topics

• Arthritis, Reactive (immunology)
• CD11b Antigen (blood)
• Cell Adhesion (immunology)
• Dose-Response Relationship, Immunologic
• HLA-B27 Antigen (blood)
• Humans
• Lipopolysaccharide Receptors (blood)
• Lipopolysaccharides (immunology)
• Neutrophils (immunology)
• Prohibitins
• Receptors, IgG (blood)
• Tumor Necrosis Factor-alpha (biosynthesis)