Proteome analysis of cerebrospinal fluid in Guillain-Barré syndrome (GBS).

Abstract

We used two-dimensional difference in-gel electrophoresis (2-D-DIGE) for proteome analysis of cerebrospinal fluid (CSF) in Guillain-Barré syndrome (GBS). Spots showing >2-fold difference between GBS and controls were analysed using MALDI-TOF mass spectrometry. Proteins that were up-regulated in GBS included haptoglobin, serine/threonine kinase 10, alpha-1-antitrypsin, SNC73, alpha II spectrin, IgG kappa chain and cathepsin D preprotein, while transferrin, caldesmon, GALT, human heat shock protein 70, amyloidosis patient HL-heart-peptide 127aa and transthyretin were down-regulated. Some of these proteins are reported in CSF of GBS for the first time. Accordingly, the 2-D-DIGE technology may be useful to identify disease-specific proteins in patients with GBS.

• Authors: V Lehmensiek, S D Süssmuth, J Brettschneider, G Tauscher, S Felk, F Gillardon, H Tumani
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 185 Issue 1-2 Pg. 190-4 (Apr 2007) ISSN: 0165-5728 [Print] Netherlands
• PMID: 17367871 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteome

Topics

• Adult
• Aged
• Cerebrospinal Fluid (chemistry)
• Electrophoresis, Gel, Two-Dimensional
• Electrophoresis, Polyacrylamide Gel
• Female
• Guillain-Barre Syndrome (cerebrospinal fluid)
• Humans
• Image Processing, Computer-Assisted
• Male
• Middle Aged
• Proteome (analysis)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization