Abstract | RATIONALE: OBJECTIVES: METHODS: Seventeen patients with interstitial lung disease in which tungsten was detected and five control subjects were studied. Detection and mapping of elements were performed with an electron probe microanalyzer equipped with a wavelength dispersive spectrometer. We immunohistochemically stained mononuclear cells, in tissue samples available from five patients, with anti-human CD4, CD8, CD20, CD68, and CD163 antibodies, and compared the distribution of positive cells with hard metal elements. MEASUREMENTS AND MAIN RESULTS: Thirteen of 17 patients were pathologically diagnosed as having giant cell interstitial pneumonia. Tungsten and cobalt were accumulated in the centrilobular fibrotic lesions, but were never found in the control lungs. CD8+ lymphocytes and CD163+ monocyte-macrophages were distributed predominantly in centrilobular fibrotic lesions around the hard metal elements. CD163+ colocalized with tungsten. Small numbers of CD8+ and CD163+ cells were also immunohistochemically shown in peribronchiolar areas and alveolar walls. CONCLUSIONS: Macrophages may phagocytose inhaled tungsten via CD163 and play an important role in forming the fibrotic lesion of hard metal lung disease with cytotoxic T lymphocytes.
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Authors | Hiroshi Moriyama, Masayoshi Kobayashi, Toshinori Takada, Takashi Shimizu, Masaki Terada, Jun-Ichi Narita, Michio Maruyama, Kouichi Watanabe, Eiichi Suzuki, Fumitake Gejyo |
Journal | American journal of respiratory and critical care medicine
(Am J Respir Crit Care Med)
Vol. 176
Issue 1
Pg. 70-7
(Jul 01 2007)
ISSN: 1535-4970 [Electronic] United States |
PMID | 17363774
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alloys
- Antigens, CD
- Antigens, Differentiation, Myelomonocytic
- CD163 antigen
- Receptors, Cell Surface
- Tungsten Compounds
- hard metal
- tungsten carbide
- Cobalt
- Tungsten
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Topics |
- Adult
- Alloys
(adverse effects)
- Antigens, CD
(immunology)
- Antigens, Differentiation, Myelomonocytic
(immunology)
- Biopsy
- CD8-Positive T-Lymphocytes
(immunology)
- Case-Control Studies
- Cobalt
(adverse effects, immunology)
- Cohort Studies
- Female
- Giant Cells, Foreign-Body
(immunology, pathology)
- Humans
- Immunohistochemistry
- Lung Diseases, Interstitial
(immunology, pathology)
- Macrophages, Alveolar
(immunology, pathology)
- Male
- Middle Aged
- Occupational Exposure
- Phagocytosis
(immunology)
- Receptors, Cell Surface
(immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
- Tungsten
(adverse effects, immunology)
- Tungsten Compounds
(adverse effects, immunology)
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