Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion.

Abstract

We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.

• Authors: Ji-Kang Fang, Subbuswamy K Prabu, Naresh B Sepuri, Haider Raza, Hindupur K Anandatheerthavarada, Domenico Galati, Joseph Spear, Narayan G Avadhani
• Journal: FEBS letters (FEBS Lett) Vol. 581 Issue 7 Pg. 1302-10 (Apr 03 2007) ISSN: 0014-5793 [Print] England
• PMID: 17349628 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Protein Subunits
• Threonine
• Serine
• Electron Transport Complex IV
• Glycine
• tricine

Topics

• Amino Acid Sequence
• Animals
• Chromatography, Liquid
• Electron Transport Complex IV (chemistry, metabolism)
• Electrophoresis, Polyacrylamide Gel
• Glycine (analogs & derivatives, chemistry)
• Heart
• Molecular Sequence Data
• Myocardial Reperfusion Injury (enzymology)
• Myocardium (enzymology)
• Phosphorylation
• Protein Conformation
• Protein Structure, Tertiary
• Protein Subunits (chemistry, metabolism)
• Rabbits
• Serine (metabolism)
• Spectrometry, Mass, Electrospray Ionization
• Threonine (metabolism)