We have previously shown that exogenous
melatonin improves the preservation of the blood-brain barrier (BBB) and neurovascular unit following
cerebral ischemia-reperfusion. Recent evidence indicates that postischemic microglial activation exaggerates the damage to the BBB. Herein, we explored whether
melatonin mitigates the cellular inflammatory response after transient focal
cerebral ischemia for 90 min in rats.
Melatonin (5 mg/kg) or vehicle was given intravenously at reperfusion onset. Immunohistochemistry and flow cytometric analysis were used to evaluate the cellular inflammatory response at 48 hr after reperfusion. Relative to controls,
melatonin-treated animals did not have significantly changed systemic cellular inflammatory responses in the bloodstream (P > 0.05).
Melatonin, however, significantly decreased the cellular inflammatory response by 41% (P < 0.001) in the ischemic hemisphere. Specifically,
melatonin effectively decreased the extent of neutrophil emigration (Ly6G-positive/CD45-positive) and macrophage/activated microglial infiltration (CD11b-positive/CD45-positive) by 51% (P < 0.01) and 66% (P < 0.01), respectively, but did not significantly alter the population composition of T lymphocyte (CD3-positive/CD45-positive; P > 0.05). This
melatonin-mediated decrease in the cellular inflammatory response was accompanied by both reduced
brain infarction and improved neurobehavioral outcome by 43% (P < 0.001) and 50% (P < 0.001), respectively. Thus,
intravenous administration of
melatonin upon reperfusion effectively decreased the emigration of circulatory neutrophils and macrophages/monocytes into the injured brain and inhibited focal microglial activation following
cerebral ischemia-reperfusion. The finding demonstrates
melatonin's inhibitory ability against the cellular inflammatory response after
cerebral ischemia-reperfusion, and further supports its pleuripotent neuroprotective actions suited either as a monotherapy or an add-on to the
thrombolytic therapy for
ischemic stroke patients.