Abstract | UNLABELLED: THE AIM: of the study--evaluation of TSH concentration and activity of HEX and its isoenzymes A and B, in serum of patients with thyroid cancer. MATERIALS AND METHODS: Blood was taken from 7 patients with thyroid cancer (6 men and 1 woman). Control consisted of 7 healthy men. In blood serum concentration of TSH was determined with immunoenzymatic method on analyzer Axsym of Abbott and expressed in microU/mL. The activity of HEX and its isoenzymes A and B was determined by method of Chatterjee et al., as modified by Zwierz et.al. Determination of HEX was performed on microplate reader ELX800 BIO-TEK. Activity of HEX, HEX A and B was expressed in pKat/mL, and specific activity in pKat/mg protein). Protein was determined by biuret method and results were expressed in mg/mL. RESULTS: Concentration of HEX A activity in serum of thyroid cancer patients is significantly higherin comparison to healthymen (p = 0.0191). Also specific activity of HEX A in serum of thyroid cancer patients is significantly higher in comparison to healthy men (p = 0.0393). CONCLUSIONS: 1. Determination of TSH concentration in serum of thyroid cancer before the operation may confirm euthyreosis. 2. Determination of HEX A activity in serum may be helpful in diagnostics of thyroid cancer.
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Authors | Piotr Zwierz, Sławomir D Szajda, Jadwiga Snarska, Zbigniew B Supronowicz, Paweł Zawadzki, Krzysztof Zwierz, Fabian Kamińsk |
Journal | Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego
(Pol Merkur Lekarski)
Vol. 21
Issue 125
Pg. 439-42
(Nov 2006)
ISSN: 1426-9686 [Print] Poland |
Vernacular Title | Stezenie hormonu tyreotropowego oraz aktywność N-acetylo-beta-D-heksozoaminidazy i jej izoenzymów A i B w surowicy chorych na raka tarczycy. |
PMID | 17345836
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Isoenzymes
- Thyrotropin
- Hexosaminidase A
- beta-N-Acetylhexosaminidases
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Topics |
- Adult
- Biomarkers, Tumor
(blood)
- Case-Control Studies
- Female
- Hexosaminidase A
- Humans
- Isoenzymes
(blood)
- Male
- Middle Aged
- Serum
(enzymology)
- Thyroid Neoplasms
(blood, enzymology)
- Thyrotropin
(blood)
- beta-N-Acetylhexosaminidases
(blood)
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