Abstract | PURPOSE: METHODS: RESULTS: A number of metalloproteinases and inhibitors are regulated in DD. The expression of 3 key collagenases, MMP1, MMP13, and MMP14 is increased significantly in the DD nodule, as is the expression of the collagen biosynthetic enzyme ADAMTS14. The expression of MMP7, an enzyme with broad substrate specificity, is increased in the DD nodule and remains equally expressed in the DD cord. TIMP1 expression is increased significantly in DD nodule compared with normal palmar fascia. CONCLUSIONS: This study measured the expression of all MMP, ADAMTS, and TIMP genes in DD. Contraction and fibrosis may result from: (1) increased collagen biosynthesis mediated by increased ADAMTS-14; (2) an increased level of TIMP-1 blocking MMP-1- and MMP-13-mediated collagenolysis; and (3) contraction enabled by MMP-14-mediated pericellular collagenolysis (and potentially MMP-7), which may escape inhibition by TIMP-1. The complete expression profile will provide a knowledge-based approach to novel therapeutics targeting these genes.
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Authors | Phillip Johnston, Adrian J Chojnowski, Rose K Davidson, Graham P Riley, Simon T Donell, Ian M Clark |
Journal | The Journal of hand surgery
(J Hand Surg Am)
Vol. 32
Issue 3
Pg. 343-51
(Mar 2007)
ISSN: 0363-5023 [Print] United States |
PMID | 17336841
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Tissue Inhibitor of Metalloproteinases
- ADAM Proteins
- ADAMTS Proteins
- ADAMTS14 protein, human
- Matrix Metalloproteinases
- Matrix Metalloproteinase 7
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Topics |
- ADAM Proteins
(metabolism)
- ADAMTS Proteins
- Adult
- Aged
- Aged, 80 and over
- Dupuytren Contracture
(enzymology)
- Fascia
(metabolism)
- Female
- Gene Expression
- Humans
- Male
- Matrix Metalloproteinase 7
(metabolism)
- Matrix Metalloproteinases
(metabolism)
- Middle Aged
- Reverse Transcriptase Polymerase Chain Reaction
- Tissue Inhibitor of Metalloproteinases
(metabolism)
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