Abstract | OBJECTIVE: METHODS: Twenty-four male Wistar rats, weighting 280-300 g, were randomly divided into three groups (n = 8). In the oleic acid group, ALI was induced by injecting oleic acid (0.2 ml/kg) intravenously. In the intervention group, the rats received oleic acid as above, but intraperitoneal injection of intermedin 1-53 (2 nmol/kg in l ml saline) was given 1 h before and 2 h after oleic acid infusion, respectively. The control group was given normal saline (0.2 ml/kg). The animals were observed for 6 h after intravenous infusion. The histopathological changes of lung tissue were studied. The arterial blood gases, lung wet/dry weight ratio (W/D), leukocyte count and neutrophil differentials in bronchoalveolar lavage fluid (BALF) were measured. Contents of intermedin 1-53 in the plasma were detected with radioimmunoassay, myeloperoxidase activity, and malondialdehyde (MDA) and conjugated diene (C-diene) level of lung tissue were also measured. RESULTS: The PaO(2) was higher and lung wet/dry weight ratio, leukocyte count and neutrophil counts in BALF were significantly lower in the intermedin group [(81.7 +/- 4.6) mm Hg (1 mm Hg = 0.133 kPa), 5.49 +/- 0.63, (57.9 +/- 7.4) x 10(4)/ml, 0.718 +/- 0.085, respectively] as compared to the oleic acid group [(60.8 +/- 3.2) mm Hg, 6.18 +/- 0.34, (122.0 +/- 16.6) x 10(4)/ml, 0.878 +/- 0.026], q value was 16.74, 3.43, 17.23, 6.32, respectively, all P < 0.05 or 0.01). CONCLUSIONS: Intermedin 1 - 53 treatment significantly attenuated oleic acid-induced lung injury in rats. Intermedin 1-53 exerts pulmo-protective effects by inhibiting formation of lipid oxide products.
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Authors | Xiao-min Yu, Xin-min Liu, Yong-fen Qi, Zhi-gang Zhang, Chao-shu Tang |
Journal | Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
(Zhonghua Jie He He Hu Xi Za Zhi)
Vol. 29
Issue 12
Pg. 808-11
(Dec 2006)
ISSN: 1001-0939 [Print] China |
PMID | 17327082
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Pyrrolizidine Alkaloids
- Oleic Acid
- Malondialdehyde
- Peroxidase
- indicine
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Topics |
- Acute Lung Injury
(chemically induced, metabolism)
- Animals
- Lung
(metabolism, pathology)
- Male
- Malondialdehyde
(metabolism)
- Oleic Acid
(adverse effects)
- Peroxidase
(metabolism)
- Pyrrolizidine Alkaloids
(blood, pharmacology)
- Rats
- Rats, Wistar
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