[Relationship between nuclear factor kappa B signal transduction way and the sensitivity of tongue carcinoma cell to Pingyangmycin chemotherapy].

Abstract

OBJECTIVE:

To examine the relationship between NF-kappaB signal transduction way and the sensitivity of Tca8113 carcinoma cell to Pingyangmycin chemotherapy.

METHODS:

2 mg/L antisense p65 oligodeoxynucle-otides (5'-GAACAGTTCGTCCATGGCCG-3') was transfected into Tca8113 cells through Lepofectin vectin, then the cells were treated with 8 mg/L Pingyangmycin. After 3 h and 6 h, the expression of p65 in nucleus was studied with immunohistochemical methods and Western blot analyses. After 48 h, the inhibitory rate of cell growth was detected with MTT assay.

RESULTS:

The NF-kappaB/p65 signal transduction way in Tca8113 cells was activated after treated with Pingyangmycin. But the transfection of antisense p65 oligodeoxynucle-otides inhibited the activation of the signal transduction, the p65 expression in nucleus was decreased significantly (P < 0.05) at 6 h, and the inhibitory rate of cell growth was increased significantly (P < 0.05) at 48 h.

CONCLUSION:

The results suggested the chemotherapeutic sensitivity of Tca8113 carcinoma cell to Pingyangmycin was improved by the inhibition of NF-kappaB/p65 signal transduction.

• Authors: Jun-Lou Wu, Xin-Hua Liang, Zu-Yi Mao, Gui-Zhou Xiao
• Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology (Hua Xi Kou Qiang Yi Xue Za Zhi) Vol. 24 Issue 5 Pg. 451-4 (Oct 2006) ISSN: 1000-1182 [Print] China
• PMID: 17315660 (Publication Type: Journal Article)

Chemical References

• NF-kappa B
• Transcription Factor RelA
• Bleomycin
• bleomycetin

Topics

• Bleomycin (analogs & derivatives)
• Cell Line, Tumor
• Cell Proliferation
• Humans
• NF-kappa B
• Signal Transduction
• Tongue Neoplasms
• Transcription Factor RelA
• Transfection