Abstract |
The protective efficacy of immunization against anthrax with Bacillus anthracis protective antigen (PA) combined with different adjuvants was tested in Hartley guinea pigs and CBA/J and A/J mice. Adjuvant components derived from microbial products that were tested included threonyl- muramyl dipeptide (threonyl-MDP); monophosphoryl lipid A (MPL); trehalose dimycolate (TDM); and the delipidated, deproteinized, cell wall skeleton (CWS) from either Mycobacterium phlei or the BCG strain of Mycobacterium bovis. Non-microbially derived adjuvants tested included aluminum hydroxide and the lipid amine CP-20,961. In guinea pigs, all adjuvants and adjuvant mixtures enhanced antibody titers to PA as well as survival after a parenteral challenge of virulent B. anthracis Ames spores. In contrast, PA alone or combined with either aluminum hydroxide or CP-20,961 failed to protect mice. Vaccines containing PA combined with threonyl-MDP or MPL-TDM-CWS protected a majority of female CBA/J mice. Statistical analysis of survival data in the guinea pigs indicated that PA-MPL-CWS and PA-MPL-TDM-CWS were more efficacious than the currently licensed human anthrax vaccine.
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Authors | B E Ivins, S L Welkos, S F Little, M H Crumrine, G O Nelson |
Journal | Infection and immunity
(Infect Immun)
Vol. 60
Issue 2
Pg. 662-8
(Feb 1992)
ISSN: 0019-9567 [Print] United States |
PMID | 1730501
(Publication Type: Journal Article)
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Chemical References |
- Adjuvants, Immunologic
- Antigens, Bacterial
- Bacterial Vaccines
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Animals
- Anthrax
(prevention & control)
- Antigens, Bacterial
(immunology)
- Bacillus anthracis
(immunology)
- Bacterial Vaccines
(administration & dosage, immunology)
- Female
- Guinea Pigs
- Immunization
- Male
- Mice
- Mice, Inbred CBA
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