Abstract | PURPOSE: MATERIALS AND METHODS: A catheter was inserted into the bladder of female mice. After urine was emptied normal saline, carbachol (100 nM) or lipopolysaccharide (10 mg/ml) was introduced with or without 10-minute pretreatment with IPD-1151T. Urine was removed after 45 minutes for histamine and tumor necrosis factor-alpha assays. The bladder was removed after 4 hours, minced into 1 mm2 pieces and cultured with or without triggers overnight for mediator release. The effect of IPD-1151T was also tested on rat skin vascular permeability as well as on purified rat peritoneal mast cells and human cord blood derived mast cells. RESULTS: CONCLUSIONS:
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Authors | W Boucher, D Kempuraj, J Cao, D Papaliodis, T C Theoharides |
Journal | The Journal of urology
(J Urol)
Vol. 177
Issue 3
Pg. 1186-90
(Mar 2007)
ISSN: 0022-5347 [Print] United States |
PMID | 17296442
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Arylsulfonates
- Histamine Antagonists
- Sulfonium Compounds
- Tumor Necrosis Factor-alpha
- suplatast tosilate
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Topics |
- Administration, Intravesical
- Animals
- Arylsulfonates
(administration & dosage, pharmacology)
- Capillary Permeability
(drug effects)
- Cell Culture Techniques
- Cystitis, Interstitial
(metabolism)
- Female
- Histamine Antagonists
(administration & dosage, pharmacology)
- Histamine Release
(drug effects)
- Humans
- Male
- Mast Cells
(drug effects)
- Mice
- Mice, Inbred C57BL
- Rats
- Rats, Sprague-Dawley
- Skin
(drug effects)
- Sulfonium Compounds
(administration & dosage, pharmacology)
- Tumor Necrosis Factor-alpha
(urine)
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