Abstract | PURPOSE: MATERIALS AND METHODS: We used randomly selected renal cancer specimens. Specimens were analyzed for coxsackie and adenovirus receptor expression using reverse transcriptase-polymerase chain reaction and immunohistochemistry. In vitro experiments on cytotoxicity were performed to determine a nontoxic dose of FK-228 for renal cancer cells. The level of coxsackie and adenovirus receptor expression was determined by fluorescence activated cell scanning and/or reverse transcriptase-polymerase chain reaction in FK-228 treated renal cancer cells. The effect in vivo on adenoviral gene expression was investigated in athymic mice. RESULTS: CONCLUSIONS: In human renal cancer specimens a loss of or decrease in coxsackie and adenovirus receptor expression may be an early event in renal cancer progression. Pretreatment with FK-228 may increase tumor cell sensitivity to adenoviral gene therapy vectors.
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Authors | Takatsugu Okegawa, Jennifer R Sayne, Kikuo Nutahara, Rey-Chen Pong, Hossain Saboorian, Wareef Kabbani, Eiji Higashihara, Jer-Tsong Hsieh |
Journal | The Journal of urology
(J Urol)
Vol. 177
Issue 3
Pg. 1148-56
(Mar 2007)
ISSN: 0022-5347 [Print] United States |
PMID | 17296436
(Publication Type: Journal Article)
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Chemical References |
- Depsipeptides
- Histone Deacetylase Inhibitors
- RNA, Messenger
- Receptors, Virus
- romidepsin
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Topics |
- Adenoviridae
- Aged
- Aged, 80 and over
- Animals
- Carcinoma, Renal Cell
(metabolism, pathology)
- Cell Culture Techniques
- Depsipeptides
(pharmacology)
- Enterovirus
- Female
- Histone Deacetylase Inhibitors
- Humans
- Kidney Neoplasms
(metabolism, pathology)
- Male
- Mice
- Mice, Nude
- Middle Aged
- RNA, Messenger
(metabolism)
- Receptors, Virus
(drug effects, genetics, metabolism)
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