Abstract | OBJECTIVE: METHODS: Intravital fluorescent microscopy was used to observe leukocyte-endothelium interaction in a "dorsal window chamber" model. Twenty-eight postcapillary veins were analyzed in group 1, and twenty-two in group 2. Group 1 received 25 mg/kg pentoxifylline 20 min before reperfusion. Group 2 received an equivalent volume of 0.9% saline at the same time. The period of ischemia was 4 h. RESULTS: Quantification of leukocyte 'sticking' and 'rolling' was done before ischemia and at 30, 60, and 120 min after reperfusion. Offline video analysis was used for evaluating the results. Statistical evaluation showed that pentoxifylline significantly attenuated leukocyte 'sticking' and 'rolling' in postcapillary venules. It was also effective in preventing 'no-reflow' when compared with the control group. CONCLUSION:
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Authors | Cigdem Unal, Cenk Sen, Deniz Iscen, Hakki Dalcik |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 138
Issue 2
Pg. 259-66
(Apr 2007)
ISSN: 0022-4804 [Print] United States |
PMID | 17275848
(Publication Type: Journal Article)
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Chemical References |
- Vasodilator Agents
- Pentoxifylline
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Topics |
- Animals
- Catheterization
- Cell Communication
(drug effects, immunology)
- Disease Models, Animal
- Endothelial Cells
(immunology, pathology)
- Leukocyte Rolling
(drug effects)
- Leukocytes
(pathology)
- Microcirculation
(drug effects, physiology)
- Microscopy, Fluorescence
(methods)
- Pentoxifylline
(pharmacology)
- Prostheses and Implants
- Rats
- Rats, Wistar
- Reperfusion Injury
(drug therapy, immunology, pathology)
- Vasodilator Agents
(pharmacology)
- Venules
(immunology, pathology)
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