Enhancement of chromosomal aberrations in tumor cells with a non-labeled Cu-PTSM and irradiation with Cu K-shell monochromatic X rays.

Abstract

This study aims to clarify the enhancement effect on chromosomal aberrations via selective energy absorption by Cu atoms in cultured bone cancer cells with non-labelled Cu-pyruvaldehyde-bis(N(4)-methylsemicarbazone) (Cu-PTSM) and monochromatic X rays. The X rays having the energy of the Cu K-shell absorption edge arising from synchrotron radiation (KEK-PF) was used as radiation source. Cu-PTSM labelled with copper radionuclides has been developed for medical imaging using positron emission tomography (PET). Evidence clearly showed that the absorption of X rays in the Cu K-shell caused this enhancement. After cells were treated with 1000 nM Cu-PTSM, the enhancement ratio of the initial yield of isochromatid breaks caused by CuK-H X-ray irradiation to that by 200 kV(p) X rays was approximately 2.8. About 5.6 times of the remaining isochromatid breaks were observed at 4 h in Cu-PTSM treated samples irradiated with CuK-H X rays comparing to that with 200 kV(p) X rays. In this study, uniqueness in physical property such as Cu atom K-shell ionisation was applied for the enhancement of biological effects in cancer cells.

• Authors: Kaoru Takakura, Satoshi Yaguchi, Yuichi Kanansugi, Katsumi Kobayashi, Ryuichi Okayasu, Yasuhisa Fujibayashi
• Journal: Radiation protection dosimetry (Radiat Prot Dosimetry) Vol. 122 Issue 1-4 Pg. 188-94 ( 2006) ISSN: 0144-8420 [Print] England
• PMID: 17234644 (Publication Type: Journal Article)

Chemical References

• Copper Radioisotopes
• DNA, Neoplasm
• Organometallic Compounds
• Thiosemicarbazones
• copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex

Topics

• Adolescent
• Bone Neoplasms (pathology, physiopathology)
• Chromosome Aberrations (radiation effects)
• Chromosomes (genetics, radiation effects)
• Copper Radioisotopes
• DNA, Neoplasm (genetics, radiation effects)
• Dose-Response Relationship, Radiation
• Female
• Humans
• Organometallic Compounds (radiation effects)
• Osteosarcoma (pathology, physiopathology)
• Radiation Dosage
• Staining and Labeling (methods)
• Thiosemicarbazones (radiation effects)
• Tumor Cells, Cultured
• X-Rays