HIV-susceptible transgenic rats allow rapid preclinical testing of antiviral compounds targeting virus entry or reverse transcription.

Abstract

The current testing of anti-HIV drugs is hampered by the lack of a small animal that is readily available and easy to handle; can be infected systemically with HIV type 1 (HIV-1); harbors the major HIV-1 target cells in a physiological frequency, organ distribution, and activation state; and is established as a pharmacological model. Here, we explored the potential of outbred Sprague-Dawley rats that transgenically express the HIV-1 receptor complex on CD4 T cells and macrophages as a model for the preclinical evaluation of inhibitors targeting virus entry or reverse transcription. The concentrations of the peptidic fusion inhibitor enfuvirtide or the nonnucleoside reverse transcriptase inhibitor efavirenz required to inhibit HIV-1 infection of cultured primary CD4 T cells and macrophages from human CD4 and CCR5-transgenic rats differed by no more than 3-fold from those required for human reference cultures. Prophylactic treatment of double-transgenic rats with a weight-adapted pediatric dosing regimen for either enfuvirtide (s.c., twice-daily) or efavirenz (oral, once-daily) achieved a 92.5% or 98.8% reduction, respectively, of the HIV-1 cDNA load in the spleen 4 days after i.v. HIV-1 challenge. Notably, a once-daily dosing regimen for enfuvirtide resulted in a approximately 5-fold weaker inhibition of infection, unmasking the unfavorable pharmacokinetic characteristics of the synthetic peptide in the context of an efficacy trial. This work provides proof of principle that HIV-susceptible transgenic rats can allow a rapid and predictive preclinical evaluation of the inhibitory potency and of the pharmacokinetic properties of antiviral compounds targeting early steps in the HIV replication cycle.

• Authors: Christine Goffinet, Ina Allespach, Oliver T Keppler
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 104 Issue 3 Pg. 1015-20 (Jan 16 2007) ISSN: 0027-8424 [Print] United States
• PMID: 17209012 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-HIV Agents
• CD4 Antigens
• Receptors, CCR5

Topics

• Animals
• Animals, Genetically Modified
• Anti-HIV Agents (therapeutic use)
• CD4 Antigens (metabolism)
• Cells, Cultured
• Disease Susceptibility
• Drug Evaluation, Preclinical
• HIV Infections (drug therapy, genetics, virology)
• HIV-1 (drug effects, physiology)
• Humans
• Macrophages (drug effects, metabolism)
• Rats
• Receptors, CCR5 (metabolism)
• Reverse Transcription (genetics)
• T-Lymphocytes (drug effects, metabolism)
• Time Factors
• Virion (metabolism)
• Virus Internalization (drug effects)