Thrombolytic efficacy of native recombinant staphylokinase on femoral artery thrombus of rabbits.

Abstract

AIM:

To investigate the thrombolytic efficacy, ideal dosage and administration of native recombinant staphylokinase (r-SAK).

METHODS:

Forty New Zealand rabbits were randomly assigned into the control, r-SAK low-dose, medial-dose, high dose, single bolus, allied therapy, recombinant streptokinase (r-SK) and urokinase (UK) groups. The right femoral artery thrombosis models were made by balloon injury, and 120 min after the injury, the thrombolytic agents were infused through the rabbits' parallel-ear vein.

RESULTS:

(1) 2 h after balloon injury, the pulse pressures of the right femoral arteries reduced to 0 or less than 10% of that of left femoral arteries in all groups; (2) after thrombolytic therapy, the pulse pressures in some of the femoral arteries markedly enhanced to more than 50% of that of left femoral arteries; (3) the reopening rates in the r-SAK medial and high dose groups were significantly higher than that of the control. The reopening rate of the same dose native r-SAK was significant higher than that of UK and r-SK; (4) the patency score of the right femoral arteries tended to be better in the r-SAK medial and high-dose groups than that of the low-dose group, and the time to reopening in the allied therapy group tended to be shorter.

CONCLUSION:

(1) r-SAK has a definite thrombolytic effect on the femoral artery thrombus of rabbits; (2) single bolus is an effective manner of r-SAK therapy, and r-SAK allied therapy with heparin may shorten the time to recanalization; (3) the efficacy of the same dose native r-SAK was superior to that of r-SK and UK.

• Authors: Chun-Jian Li, Jun Huang, Zhi-Jian Yang, Ke-Jiang Cao
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 28 Issue 1 Pg. 58-65 (Jan 2007) ISSN: 1671-4083 [Print] United States
• PMID: 17184583 (Publication Type: Journal Article)

Chemical References

• Fibrinolytic Agents
• Recombinant Proteins
• Heparin
• Metalloendopeptidases
• auR protein, Staphylococcus aureus

Topics

• Animals
• Blood Pressure (drug effects)
• Dose-Response Relationship, Drug
• Drug Therapy, Combination
• Femoral Artery (drug effects, pathology, physiopathology)
• Fibrinolytic Agents (administration & dosage, therapeutic use)
• Heparin (therapeutic use)
• Male
• Metalloendopeptidases (administration & dosage, genetics, therapeutic use)
• Rabbits
• Random Allocation
• Recombinant Proteins (administration & dosage, therapeutic use)
• Thrombosis (drug therapy, pathology, physiopathology)
• Treatment Outcome