In
hemodialysis subjects correction of
anemia is facilitated by combined supplementation of intravenous
iron and recombinant human
erythropoietin. Reticulocyte
hemoglobin content (RET-He) is considered to be an actual
indicator reflecting functional
iron availability for erythropoiesis. In the present study, interdependence between biochemical analytes reflecting
iron status and hemocytometric parameters indicating the degree of hemoglobinization of reticulocytes and red blood cells, respectively, is established. Participants of the study were reference subjects (n=75), subjects with
iron deficiency anemia (n=52), subjects with
uremia (n=19) and subjects undergoing
hemodialysis treatment (n=43). If compared with the reference subjects the results for RBC counts and MCHC are statistically significantly decreased in case of subjects with
hemodialysis and
uremia, whereas increased results are established with regard to RDW-sd values. Significantly increased results for absolute reticulocyte counts and immature reticulocyte fractions (IRF) are also observed in case of subjects with
hemodialysis and
uremia. Slightly increased values for the ZPP/
heme ratio in combination with elevated reticulocyte count reflect increased activity of erythropoiesis. At a definite MCV value, decreased levels for the
hemoglobin content of reticulocytes (RET-He) and
hemoglobin content of red blood cells (RBC-He) are observed in case of subjects treated with
hemodialysis and in subjects with
uremia if compared with identical MCV values of the group of reference subjects. For the ratio of RET-He and RBC-He obviously decreased results are demonstrated in case of subjects with
iron deficiency anemia (1.02 +/- 0.08, mean +/- SD),
hemodialysis (1.05 +/- 0.05) and
uremia (1.02 +/- 0.10) if compared with the group of reference subjects (1.11 +/- 0.02). From the combined interpretation of the MCV values within the reference range and decreased values for RET-He and RET-He/RBC-He ratios, respectively, a decreased degree of hemoglobinization is concluded in the case of subjects with
hemodialysis or
uremia. The conclusion implicating the presumption of reduced functional availability of
iron for
hemoglobin synthesis is supported by the detection of increased results for sTfR concentrations and ZPP/
heme ratios.