The association of VP1 unique region protein in acute parvovirus B19 infection and anti-phospholipid antibody production.

Abstract

BACKGROUND:

Previous studies have postulated a connection between human parvovirus B19 (B19) infection and anti-phospholipid antibodies (aPL). Recently, the phospholipase domain of B19 has been linked to B19-VP1 unique region (VP1u). To elucidate the roles of VP1u in B19 infection and aPL production, the major reactivity of anti-B19-VP1u, anti-cardiolipin antibody (aCL), and anti-beta2-glycoprotein I (beta2GPI) antibody was evaluated.

METHODS:

Sera from 102 clinically suspected cases of B19 infection were analyzed by nested PCR and ELISA. Humoral responses of anti-B19-VP1u and anti-B19-VP1uD175A IgM/IgG antibodies, aCL and the anti-beta2GPI antibody were assessed by Western blot and ELISA. Absorption experiments were also performed to determine the binding specificity of immunoglobulins to B19-VP1u, CL and beta2GPI.

RESULTS:

Sera from patients with the diagnostic pattern DNA+/IgM+/IgG+ had a high frequency (57%) for recognition of CL and beta2GPI. Furthermore, adsorption experiments were performed by adding purified B19-VP1u, which partially suppressed the reactivity of anti-B19VP1u to CL and beta2GPI.

CONCLUSIONS:

Serum from patients with acute B19 infection has a high frequency in recognition of CL and beta2GPI, and the phospholipase domain observed in the B19-VP1u may have contributed to the production of aPL. These findings may provide a clue for understanding the roles of B19-VP1u in B19 infection and aPL production.

• Authors: Bor-Show Tzang, Gregory J Tsay, Yi-Ju Lee, Chin Li, Yu-Shu Sun, Tsai-Ching Hsu
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 378 Issue 1-2 Pg. 59-65 (Mar 2007) ISSN: 0009-8981 [Print] Netherlands
• PMID: 17169353 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Antiphospholipid
• Antibodies, Viral
• Capsid Proteins
• Cardiolipins
• DNA, Viral
• Immunoglobulin G
• Immunoglobulin M
• beta 2-Glycoprotein I
• capsid protein VP1, parvovirus B19

Topics

• Adolescent
• Adult
• Aged
• Antibodies, Antiphospholipid (biosynthesis)
• Antibodies, Viral (blood)
• Capsid Proteins (genetics, immunology)
• Cardiolipins (blood)
• Child
• DNA, Viral (blood)
• Female
• Humans
• Immunoglobulin G (blood)
• Immunoglobulin M (blood)
• Male
• Middle Aged
• Parvoviridae Infections (immunology)
• Parvovirus B19, Human (immunology)
• beta 2-Glycoprotein I (immunology)