[Electrophoretic analysis of lacrimal proteins--a diagnostic method in secondary Sjogren syndrome].

Abstract

PURPOSE:

We analysed and compared electrophoretic tear proteins patterns of healthy subjects and patients with different autoimmune diseases associated with secondary Sjogren's syndrome.

MATERIALS AND METHODS:

Tears were collected using the Schirmer's method. Proteins were separated by sodium-dodecyl sulfate polyacrylamide gel electrophoresis. The lanes were stained by Coomassie blue and/or silver.

RESULTS:

Lactoferrin, albumin, lipocalin and lysozyme were found to be the main components being identified using molecular weight markers.

CONCLUSIONS:

Electrophoretic analysis of tear proteins patterns is a fast, reproducible and simple method which provides information about the possibility of lacrimal gland involvement in auto-immune diseases.

• Authors: Viorica Madalina Cojocaru, Coziana Ciurtin, Elena Puchianu, Felicia Anothe
• Journal: Oftalmologia (Bucharest, Romania : 1990) (Oftalmologia) Vol. 50 Issue 3 Pg. 3-5 ( 2006) ISSN: 1220-0875 [Print] Romania
• Vernacular Title: Analiza electroforetică a proteinelor lacrimale - metodă de diagnostic a sindromului Sjogren secundar.
• PMID: 17144498 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Anti-Infective Agents
• Carrier Proteins
• Cysteine Proteinase Inhibitors
• Eye Proteins
• LCN1 protein, human
• Lipocalin 1
• Muramidase
• Lactoferrin

Topics

• Anti-Infective Agents (analysis)
• Autoimmune Diseases (diagnosis)
• Carrier Proteins (analysis)
• Cysteine Proteinase Inhibitors (analysis)
• Electrophoresis, Gel, Two-Dimensional
• Eye Proteins (analysis)
• Humans
• Lactoferrin (analysis)
• Lipocalin 1
• Muramidase (analysis)
• Sjogren's Syndrome (diagnosis, immunology)
• Tears (chemistry)