Abstract | BACKGROUND: PURPOSE: The purpose of this study was to investigate the presence of HPV DNA in children with benign tonsillar and/or adenoid hyperplasia. METHODS: One hundred six samples of paraffin-embedded adenoid and/or tonsillar tissues from 102 children, 57 girls and 45 boys (age range, 2-14 years), were tested for the presence of HPV DNA using polymerase chain reaction (PCR) with general primers GP5+/GP6+. HPV typing was performed by PCR with specific primers for HPV-16, -18, -33 and -11. RESULTS: HPV DNA was detected in 9 (8.5%) of the 106 collected specimens. The frequencies of HPV typing were 6 of 9 (66.7%) for HPV-16, 2 of 9 (22.2%) for HPV-11, zero of 9 (0%) for HPV-33 and HPV-18, whereas one HPV-positive sample remained untyped. No multiple HPV infection was detected. HPV was detected in 6 (9.4%) children with tonsillar hyperplasia and in 3 (7.1%) with adenoid hyperplasia. The mean age of children with HPV-positive specimens was lower than that of HPV-negative children (P = 0.006). No statistical correlation in the prevalence of HPV infection was observed according to children's sex, origin or residence (urban or rural). CONCLUSION: Although the significance of the presence of HPV DNA in tonsillar and adenoid hyperplasias remains obscure, the PCR detection of high-risk HPV DNA should be evaluated cautiously.
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Authors | Ioannis N Mammas, George Sourvinos, Catherine Michael, Demetrios A Spandidos |
Journal | The Pediatric infectious disease journal
(Pediatr Infect Dis J)
Vol. 25
Issue 12
Pg. 1158-62
(Dec 2006)
ISSN: 0891-3668 [Print] United States |
PMID | 17133162
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adenoids
(virology)
- Adolescent
- Age Factors
- Child
- Child, Preschool
- DNA Primers
- DNA, Viral
(analysis)
- Female
- Humans
- Hyperplasia
(virology)
- Male
- Palatine Tonsil
(pathology, virology)
- Papillomaviridae
(classification, genetics, isolation & purification)
- Papillomavirus Infections
(virology)
- Paraffin Embedding
- Polymerase Chain Reaction
- Residence Characteristics
- Sex Factors
- Statistics as Topic
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