Abstract | OBJECTIVE: To observe the therapeutic effect of CDglyTK gene mediated by radiation-inducible promoters in the treatment of buccal carcinoma in Golden Hamster. METHODS: Animal models of buccal carcinoma in golden hamster were established by painting 0.5% dimethyl- benzanthracene. The plasmids pcDNA (+) 3.1/E-CDglyTK were transfected into tumors by lipofectamine. 24 h later, the tumors were exposed to 3 Gy irradiation. Animals were monitored at regular intervals for volume of tumors. CDglyTK mRNA was assayed by RT-PCR. Apoptosis and proliferating cell nuclear antigen were detected respectively by in situ end-labeling and immunohistochemical methods. RESULTS: Compared with control groups, the tumor was suppressed obviously by CDglyTK gene therapy combined with 3 Gy induction radiation. The expression of CDglyTK gene could be detected by RT-PCR in the transfected tumor, and up-regulation of CDglyTK expression was found in tumor exposed to radiation (P < 0.05). There was significant difference in apoptosis index or proliferation index between tumor without irradiation and tumor with irradiation (P < 0.05). CONCLUSIONS: The radiation-inducible promoter can be served as a molecular switch to regulate the expression of CDglyTK gene in buccal carcinoma in golden hamster, and low dose induction radiation can significantly improve the therapeutic effects.
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Authors | Dong-sheng Yu, Hong-zhang Huang, Xiao-wen Hu, Xi-qiang Liu, Hai-kuo Tang, An-xun Wang |
Journal | Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
(Zhonghua Kou Qiang Yi Xue Za Zhi)
Vol. 41
Issue 9
Pg. 549-52
(Sep 2006)
ISSN: 1002-0098 [Print] China |
PMID | 17129429
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Thymidine Kinase
- Cytosine Deaminase
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Topics |
- Animals
- Carcinoma, Squamous Cell
(diagnostic imaging, therapy)
- Cheek
(diagnostic imaging)
- Cricetinae
- Cytosine Deaminase
(genetics)
- Genes, Transgenic, Suicide
(genetics)
- Genetic Therapy
(methods)
- Mesocricetus
- Mouth Neoplasms
(radiotherapy, therapy)
- Promoter Regions, Genetic
(radiation effects)
- Radiography
- Simplexvirus
(enzymology)
- Thymidine Kinase
(genetics)
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