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[Radiation-inducible promoters-mediated cdglytk gene in the treatment of buccal carcinoma in golden hamster].

AbstractOBJECTIVE:
To observe the therapeutic effect of CDglyTK gene mediated by radiation-inducible promoters in the treatment of buccal carcinoma in Golden Hamster.
METHODS:
Animal models of buccal carcinoma in golden hamster were established by painting 0.5% dimethyl-benzanthracene. The plasmids pcDNA (+) 3.1/E-CDglyTK were transfected into tumors by lipofectamine. 24 h later, the tumors were exposed to 3 Gy irradiation. Animals were monitored at regular intervals for volume of tumors. CDglyTK mRNA was assayed by RT-PCR. Apoptosis and proliferating cell nuclear antigen were detected respectively by in situ end-labeling and immunohistochemical methods.
RESULTS:
Compared with control groups, the tumor was suppressed obviously by CDglyTK gene therapy combined with 3 Gy induction radiation. The expression of CDglyTK gene could be detected by RT-PCR in the transfected tumor, and up-regulation of CDglyTK expression was found in tumor exposed to radiation (P < 0.05). There was significant difference in apoptosis index or proliferation index between tumor without irradiation and tumor with irradiation (P < 0.05).
CONCLUSIONS:
The radiation-inducible promoter can be served as a molecular switch to regulate the expression of CDglyTK gene in buccal carcinoma in golden hamster, and low dose induction radiation can significantly improve the therapeutic effects.
AuthorsDong-sheng Yu, Hong-zhang Huang, Xiao-wen Hu, Xi-qiang Liu, Hai-kuo Tang, An-xun Wang
JournalZhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 41 Issue 9 Pg. 549-52 (Sep 2006) ISSN: 1002-0098 [Print] China
PMID17129429 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Thymidine Kinase
  • Cytosine Deaminase
Topics
  • Animals
  • Carcinoma, Squamous Cell (diagnostic imaging, therapy)
  • Cheek (diagnostic imaging)
  • Cricetinae
  • Cytosine Deaminase (genetics)
  • Genes, Transgenic, Suicide (genetics)
  • Genetic Therapy (methods)
  • Mesocricetus
  • Mouth Neoplasms (radiotherapy, therapy)
  • Promoter Regions, Genetic (radiation effects)
  • Radiography
  • Simplexvirus (enzymology)
  • Thymidine Kinase (genetics)

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