HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Effects of nitric oxide on expressions of nitrosocysteine and calcium-activated potassium channels in the supraoptic nuclei and neural lobe of dehydrated rats.

Abstract
Nitric oxide (NO) is an important gas mediator in the signal transduction cascade regulating osmotic function in the hypothalamo-neurohypophysial system. We previously found that increased nitric oxide synthase (NOS) activity in the supraoptic nuclei (SON) and neural lobe following osmotic stimulation and NO could regulate the expression of Ca(2+)-activated K(+) channel (BK channels) protein in the magnocellular system during dehydration. The aim of the current study is to examine the role of NO in the regulation of nitrosocysteine and BK channel protein in the magnocellular system in dehydrated animals. Using Western blot analysis and quantitative immunofluorescent staining study, we found that water deprivation in rats significantly enhanced the expression of nitrosocysteine protein in SON and neural lobes. Immunohistochemistry study indicated that dehydration significantly increased the profiles of SON neurons co-expressing nitrosocysteine with BK-channel protein. Intracerebroventricular administration of L-NAME (an inhibitor of NO synthase) significantly reduced the neuronal profiles of nitrosocysteine, as well as their co-expression with BK-channel in SON of dehydrated rats. However, treatment of sodium nitroprusside (a donor of NO) increased this co-expression. Our results indicate that NO signaling cascade may control the expression of BK channels through the regulation of nitrosocysteine in SON and neural lobe of rats during osmotic regulation.
AuthorsMassako Kadekaro, Guangxiao Su, Rong Chu, Yongzhong Lei, Junfa Li, Li Fang
JournalNeuroscience letters (Neurosci Lett) Vol. 411 Issue 2 Pg. 117-22 (Jan 10 2007) ISSN: 0304-3940 [Print] Ireland
PMID17098363 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • Enzyme Inhibitors
  • Nitric Oxide Donors
  • Potassium Channels, Calcium-Activated
  • S-Nitrosothiols
  • Nitroprusside
  • Nitric Oxide
  • S-nitrosocysteine
  • Cysteine
  • NG-Nitroarginine Methyl Ester
Topics
  • Analysis of Variance
  • Animals
  • Blotting, Western (methods)
  • Cell Count
  • Cysteine (analogs & derivatives, metabolism)
  • Dehydration
  • Enzyme Inhibitors (pharmacology)
  • Fluorescent Antibody Technique (methods)
  • Gene Expression (drug effects, physiology)
  • Male
  • NG-Nitroarginine Methyl Ester (pharmacology)
  • Nitric Oxide (pharmacology)
  • Nitric Oxide Donors (pharmacology)
  • Nitroprusside (pharmacology)
  • Pituitary Gland, Posterior (drug effects)
  • Potassium Channels, Calcium-Activated (metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • S-Nitrosothiols (metabolism)
  • Supraoptic Nucleus (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: