Abstract | BACKGROUND: METHODS: Tissue microarrays were constructed from the tumors of 56 patients with ampullary cancer undergoing pancreaticoduodenectomy. Immunohistochemical staining for osteonectin was undertaken and compared with staining in chronic pancreatitis (n = 13) and normal pancreas (n = 19). Survival curves were created by the Kaplan-Meier method and compared by log rank analysis. Median follow-up for all living patients with ampullary cancer was 69.6 months. RESULTS:
Osteonectin was significantly (P < .05, Fisher's exact test) overexpressed in the stroma of ampullary cancers (90%) relative to chronic pancreatitis (62%) and normal pancreas (0%). Tumors expressing osteonectin were more likely to have nodal metastases than those lacking osteonectin expression (48% vs. 0%, P = .06, Fisher's exact test) and showed decreased survival. Node-negative status, pylorus preservation at the time of pancreaticoduodenectomy, and lack of osteonectin expression were predictors of prolonged survival by multivariate analysis. CONCLUSIONS: Although the importance of tumor-stroma interactions in periampullary cancers is not fully understood, our data suggest that osteonectin is an integral stromal element in ampullary cancers, and its overexpression is associated with decreased survival.
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Authors | Mark Bloomston, E Christopher Ellison, Peter Muscarella, Osama Al-Saif, Edward W Martin, W Scott Melvin, Wendy L Frankel |
Journal | Annals of surgical oncology
(Ann Surg Oncol)
Vol. 14
Issue 1
Pg. 211-7
(Jan 2007)
ISSN: 1068-9265 [Print] United States |
PMID | 17080236
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- MUC2 protein, human
- Mucin-1
- Mucin-2
- Mucins
- Osteonectin
- Osteopontin
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Topics |
- Adenocarcinoma
(chemistry, mortality)
- Adult
- Aged
- Aged, 80 and over
- Ampulla of Vater
- Biomarkers, Tumor
(analysis)
- Common Bile Duct Neoplasms
(chemistry, mortality)
- Female
- Humans
- Immunohistochemistry
- Male
- Microarray Analysis
- Middle Aged
- Mucin-1
(analysis)
- Mucin-2
- Mucins
(analysis)
- Osteonectin
(analysis)
- Osteopontin
(analysis)
- Pancreaticoduodenectomy
- Survival Rate
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