Eotaxin-2 in sputum cell culture to evaluate asthma inflammation.

Abstract

The aim of the present study was to elucidate whether the culture of cells recovered from induced sputum may represent a suitable model to evaluate cytokine and chemokine production by airway inflammatory cells. Sputum induction was performed in 21 normal subjects and 30 asthmatic patients. A total of 21 out of the 30 asthmatic patients were taking inhaled corticosteroids, while the remaining nine were steroid-naive asthmatics. The steroid-naive group was evaluated before and after a 14-day treatment with oral prednisone (40 mg.day(-1)). The supernatant of lysed and centrifuged sputum and the supernatant of sputum cell culture were analysed. Tumour necrosis factor-alpha, interleukin (IL)-8 (CXCL8), IL-1beta, IL-13 and eotaxin-2 (CCL24) concentrations were determined by specific ELISA. Eotaxin-2 production by cell culture was higher in the asthma group (131+/-108 pg.mL(-1)) than in the control group (36+/-41 pg.mL(-1)) and treatment with oral corticosteroids eliminated this difference. In addition, reduction of eotaxin-2 levels by corticosteroid treatment was greater in cell culture (81.3% reduction) than in sputum (26.4%). There was correlation between the decrease in eotaxin-2 production and the decrease in blood eosinophil number and between eotaxin-2 and eosinophils in sputum. Eotaxin-2 may play an important role in asthma and the response to corticosteroid treatment suggests that analysis of sputum cell culture is relevant as an inflammatory parameter.

• Authors: M E Scheicher, M M Teixeira, F Q Cunha, A L Teixeira Jr, J T Filho, E O Vianna
• Journal: The European respiratory journal (Eur Respir J) Vol. 29 Issue 3 Pg. 489-95 (Mar 2007) ISSN: 0903-1936 [Print] England
• PMID: 17079258 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• CCL24 protein, human
• Chemokine CCL24
• Chemokines
• Chemokines, CC
• Cytokines
• Interleukin-13
• Interleukin-1beta
• Interleukin-8
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha
• Budesonide
• Prednisone

Topics

• Administration, Inhalation
• Administration, Oral
• Adult
• Anti-Inflammatory Agents (therapeutic use)
• Asthma (drug therapy, immunology)
• Budesonide (therapeutic use)
• Cells, Cultured
• Chemokine CCL24
• Chemokines (metabolism)
• Chemokines, CC (metabolism)
• Cytokines (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Eosinophils (drug effects, immunology)
• Female
• Forced Expiratory Volume (drug effects, physiology)
• Humans
• Interleukin-13 (metabolism)
• Interleukin-1beta (metabolism)
• Interleukin-8 (metabolism)
• Leukocyte Count
• Lipopolysaccharides (immunology)
• Male
• Middle Aged
• Prednisone (therapeutic use)
• Reference Values
• Saliva (immunology)
• Statistics as Topic
• Tumor Necrosis Factor-alpha (metabolism)