Abstract |
We have demonstrated before that exposure of neuronal cultures to poisoning by iodoacetic acid (IAA) followed by "reperfusion" ( IAA-R insult), results in severe cytotoxicity, which could be markedly attenuated by prior activation of the adenosine A1 receptors. We also have demonstrated that adenosine activates a signal transduction pathway (STP), which involves activation of PKC epsilon and opening of KATP channels. Here, we provide proof for the involvement also of phospholipase C (PLC) in the neuronal protective adenosine-activated STP. R-PIA, a specific A1 adenosine receptor agonist, was found to enhance neuronal PLC activity and protect against the IAA-R insult. The PLC inhibitor U73122, abrogated both R-PIA-induced effects. These results demonstrate that activation of PLC is a vital step in the neuronal protective adenosine-induced STP.
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Authors | A Rogel, Y Bromberg, O Sperling, E Zoref-Shani |
Journal | Nucleosides, nucleotides & nucleic acids
(Nucleosides Nucleotides Nucleic Acids)
Vol. 25
Issue 9-11
Pg. 1283-6
( 2006)
ISSN: 1525-7770 [Print] United States |
PMID | 17065107
(Publication Type: Journal Article)
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Chemical References |
- Enzyme Inhibitors
- Estrenes
- Neuroprotective Agents
- Pyrrolidinones
- 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
- Type C Phospholipases
- Adenosine
- Iodoacetic Acid
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Topics |
- Adenosine
(metabolism)
- Animals
- Brain
(embryology)
- Enzyme Activation
- Enzyme Inhibitors
(pharmacology)
- Estrenes
(pharmacology)
- Iodoacetic Acid
(pharmacology)
- Neurons
(metabolism)
- Neuroprotective Agents
(pharmacology)
- Pyrrolidinones
(pharmacology)
- Rats
- Reperfusion Injury
- Signal Transduction
- Type C Phospholipases
(metabolism)
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