Neural transplantation of human MSC and NT2 cells in the twitcher mouse model.

Abstract

BACKGROUND:

Accumulating evidence has demonstrated that the NT2 embryonal carcinoma cell line and multipotential stem cells found in BM, mesenchymal stromal cells (MSC), have the ability to differentiate into a wide variety of cell types. This study was designed to explore the efficacy of these two human stem cell types as a graft source for the treatment of demyelinating disorders such as Krabbe's disease and multiple sclerosis (MS).

METHODS:

We examined the engraftment and in vivo differentiation of adult MSC and NT2 cells after transplantation into two demyelinating environments, the neonatal and postnatal twitcher mouse brain.

RESULTS:

Both types of xenografts led to anatomical integration, without tumor formation, and remained viable in the normal and twitcher mouse brain, showing differentiation into neurons, astrocytes and oligodendrocytes.

DISCUSSION:

This study represents a platform for further stem cell transplantation studies in the twitcher model and potentially has important therapeutic implications.

• Authors: J Croitoru-Lamoury, K R Williams, F M J Lamoury, L A Veas, B Ajami, R M Taylor, B J Brew
• Journal: Cytotherapy (Cytotherapy) Vol. 8 Issue 5 Pg. 445-58 ( 2006) ISSN: 1465-3249 [Print] England
• PMID: 17050249 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Astrocytes (metabolism, pathology)
• Brain (metabolism, pathology)
• Brain Neoplasms (metabolism, pathology)
• Cell Differentiation
• Cell Line, Tumor
• Disease Models, Animal
• Humans
• Leukodystrophy, Globoid Cell (metabolism, pathology, therapy)
• Mesoderm (metabolism, pathology)
• Mice
• Mice, Mutant Strains
• Multipotent Stem Cells (metabolism, pathology)
• Neoplasm Transplantation (methods)
• Neoplasms, Experimental (metabolism, pathology)
• Neurons (metabolism, pathology)
• Oligodendroglia (metabolism, pathology)
• Stem Cell Transplantation
• Transplantation, Heterologous