Abstract |
The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.
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Authors | Markus Mueller, Sebastian Bunk, Isabel Diterich, Michael Weichel, Carolin Rauter, Dieter Hassler, Corinna Hermann, Reto Crameri, Thomas Hartung |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 44
Issue 10
Pg. 3778-80
(Oct 2006)
ISSN: 0095-1137 [Print] United States |
PMID | 17021109
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Peptide Library
- Ribosomal Proteins
- ribosomal protein L25
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Topics |
- Adult
- Borrelia burgdorferi
(genetics)
- Humans
- Lyme Disease
(diagnosis)
- Middle Aged
- Peptide Library
- Ribosomal Proteins
(genetics)
- Sensitivity and Specificity
- Serologic Tests
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