Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis.

Abstract

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

• Authors: Markus Mueller, Sebastian Bunk, Isabel Diterich, Michael Weichel, Carolin Rauter, Dieter Hassler, Corinna Hermann, Reto Crameri, Thomas Hartung
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 44 Issue 10 Pg. 3778-80 (Oct 2006) ISSN: 0095-1137 [Print] United States
• PMID: 17021109 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peptide Library
• Ribosomal Proteins
• ribosomal protein L25

Topics

• Adult
• Borrelia burgdorferi (genetics)
• Humans
• Lyme Disease (diagnosis)
• Middle Aged
• Peptide Library
• Ribosomal Proteins (genetics)
• Sensitivity and Specificity
• Serologic Tests