Abstract |
Interleukin-18 (IL-18) has multiple effects on various cells that are involved in immune escape of murine melanoma cells and in the inflammatory responses. This study investigated the effect of IL-18 on the ability of murine melanoma cells to migrate by using B16F10 cells and the IL-18 antisense transfectants of B16F10 cells (the B16F10/IL-18 antisense transfectant). The B16F10 cells were more able to migrate than were the B16F10/IL-18 antisense transfectants. An exogenous IL-18 treatment improved the ability of the B16F10/IL-18 antisense transfectant cells to migrate, indicating that IL-18 enhanced the migration ability of melanoma cells. To determine the signaling mechanisms involved in IL-18-enhanced migration, we measured the ROI levels. It was found that the ROI levels were increased by IL-18, and an antioxidant, N-acetyl-l-cystein (NAC), blocked the effect of IL-18 on migration, suggesting the involvement of ROI in the signal transduction of IL-18-enhanced cell migration. IL-18-enhanced cell migration was also reduced by PD98059. In addition, the level of ERK1/2 phosphorylation was markedly increased by treating with exogenous IL-18 at 20 min. These results suggest that IL-18 enhances the ability of melanoma cells to migrate via the generation of ROI and the MAPK pathway.
|
Authors | Min Kyung Jung, Hyun Keun Song, Kyung-Eun Kim, Dae Young Hur, Taesung Kim, Saic Bang, Hyunjeong Park, Dae Ho Cho |
Journal | Immunology letters
(Immunol Lett)
Vol. 107
Issue 2
Pg. 125-30
(Nov 15 2006)
ISSN: 0165-2478 [Print] Netherlands |
PMID | 17014914
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Flavonoids
- Free Radical Scavengers
- Interleukin-18
- Protein Kinase Inhibitors
- Reactive Oxygen Species
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinases
- 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
- Acetylcysteine
|
Topics |
- Acetylcysteine
(pharmacology)
- Animals
- Cell Movement
(drug effects)
- Flavonoids
(pharmacology)
- Free Radical Scavengers
(pharmacology)
- Interleukin-18
(genetics, pharmacology, physiology)
- Melanoma, Experimental
(immunology, pathology)
- Mice
- Mitogen-Activated Protein Kinase 1
(antagonists & inhibitors, metabolism)
- Mitogen-Activated Protein Kinase 3
(antagonists & inhibitors, metabolism)
- Mitogen-Activated Protein Kinases
(antagonists & inhibitors, metabolism)
- Phosphorylation
- Protein Kinase Inhibitors
(pharmacology)
- Reactive Oxygen Species
(analysis, antagonists & inhibitors, metabolism)
- Transfection
|