Immunohistochemical and immunoblotting detection of cytokeratin in smooth muscle tumors.

Abstract

Forty-six smooth muscle tumors, including 35 of gastrointestinal origin, were studied immunohistochemically for the localization of cytokeratin using a variety of monoclonal antibodies. In formalin-fixed, paraffin-embedded material, six of 40 leiomyomas, two of five leiomyosarcomas and one leiomyoblastoma were immunoreactive for cytokeratin in a few tumor cells. A proportion of non-neoplastic smooth muscle cells also exhibited a positive reaction. In fresh frozen sections of one gastric leiomyosarcoma, a high percentage of tumor cells were reactive with nine of the eleven anti-cytokeratin monoclonal antibodies examined. Cytokeratin-positive cytoplasmic filaments were further demonstrated by immunoelectron microscopy. By immunoblot analysis, an extract of this immunohistochemically cytokeratin-positive leiomyosarcoma showed a distinct band at the same position as an extract of pancreas, whereas no bands were seen in an extract of a cytokeratin-negative esophageal leiomyosarcoma. These immunohistochemical and immunoblotting findings indicate that a certain subset of smooth muscle neoplasms express genuine cytokeratin filaments.

• Authors: K Tauchi, Y Tsutsumi, S Yoshimura, K Watanabe
• Journal: Acta pathologica japonica (Acta Pathol Jpn) Vol. 40 Issue 8 Pg. 574-80 (Aug 1990) ISSN: 0001-6632 [Print] Australia
• PMID: 1700573 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Keratins

Topics

• Humans
• Immunoblotting (methods)
• Immunohistochemistry (methods)
• Keratins (metabolism)
• Leiomyoma (metabolism, pathology)
• Leiomyosarcoma (metabolism, pathology)
• Microscopy, Electron (methods)
• Neoplasms, Muscle Tissue (metabolism, pathology)