BP1 is a negative modulator of definitive erythropoiesis.

Abstract

Beta protein 1 (BP1), a human homeotic transcription factor, is expressed during hematopoeisis in the erythroid lineage. To determine the in vivo role of BP1 in erythropoiesis, we have undertaken two complementary approaches using enforced BP1 expression in both transgenic mice and embryonic stem (ES) cells. Despite repeated attempts, only one adult transgenic BP1 founder mouse among 121 mice was obtained. This mouse presumably survived due to transgene mosaicism because the transgene could not be transmitted. This mouse expressed BP1 and displayed splenomegaly, extramedullary erythropoiesis and severe amyloidosis A in the kidney, a phenotype compatible with thalassemia. Consistently, the presence of BP1 transgene in fetuses was associated with paleness and lethality. In ES cells, BP1 expression in primary differentiation appeared to antagonize adult beta-globin expression. In secondary differentiation, BP1 expression reduced significantly beta-globin gene expression in both primitive and definitive erythroid cells, whereas it impaired only the definitive erythroid cell differentiation. These studies showed that BP1 can negatively modulate adult beta-globin gene expression and definitive erythroid cell differentiation, and suggest that BP1 could play a role in thalassemia.

• Authors: Marthe-Sandrine Eiymo Mwa Mpollo, Mélissa Beaudoin, Patricia E Berg, Hugues Beauchemin, Vivette D'Agati, Marie Trudel
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 34 Issue 18 Pg. 5232-7 ( 2006) ISSN: 1362-4962 [Electronic] England
• PMID: 17003054 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DLX4 protein, human
• Homeodomain Proteins
• Transcription Factors
• Globins

Topics

• Amyloidosis (pathology)
• Animals
• Cell Line
• Embryonic Stem Cells (metabolism)
• Erythroid Precursor Cells (metabolism)
• Erythropoiesis
• Genes, Lethal
• Globins (genetics)
• Homeodomain Proteins (genetics, metabolism, physiology)
• Humans
• Kidney Diseases (pathology)
• Mice
• Mice, Transgenic
• Transcription Factors (genetics, metabolism, physiology)