Abstract |
Postprandial hypertriglyceridemia and low plasma HDL levels, which are principal features of the metabolic syndrome, are displayed by transgenic mice expressing human apolipoprotein A-II (hapoA-II). In these mice, hypertriglyceridemia results from the inhibition of lipoprotein lipase and hepatic lipase activities by hapoA-II carried on VLDL. This study aimed to determine whether the association of hapoA-II with triglyceride-rich lipoproteins (TRLs) is sufficient to impair their catabolism. To measure plasma TRL residence time, intestinal TRL production was induced by a radioactive oral lipid bolus. Radioactive and total triglyceride (TG) were rapidly cleared in control mice but accumulated in plasma of transgenic mice, in relation to hapoA-II concentration. Similar plasma TG accumulations were measured in transgenic mice with or without endogenous apoA-II expression. HapoA-II (synthesized in liver) was detected in chylomicrons (produced by intestine). The association of hapoA-II with TRL in plasma was further confirmed by the absence of hapoA-II in chylomicrons and VLDL of transgenic mice injected with Triton WR 1339, which prevents apolipoprotein exchanges. We show that the association of hapoA-II with TRL occurs in the circulation and induces postprandial hypertriglyceridemia.
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Authors | Sonia Dugué-Pujol, Xavier Rousset, Danièle Pastier, Nhuan Tran Quang, Virginie Pautre, Jean Chambaz, Michèle Chabert, Athina-Despina Kalopissis |
Journal | Journal of lipid research
(J Lipid Res)
Vol. 47
Issue 12
Pg. 2631-9
(Dec 2006)
ISSN: 0022-2275 [Print] United States |
PMID | 16990646
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoprotein A-II
- Chylomicrons
- Lipoproteins
- Lipoproteins, VLDL
- Recombinant Proteins
- Triglycerides
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Topics |
- Animals
- Apolipoprotein A-II
(blood, deficiency, genetics)
- Chylomicrons
(metabolism)
- Female
- Humans
- Hyperglycemia
(blood, etiology)
- Intestinal Mucosa
(metabolism)
- Lipoproteins
(blood)
- Lipoproteins, VLDL
(blood)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Mice, Transgenic
- Recombinant Proteins
(blood, genetics)
- Triglycerides
(blood)
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