Abstract |
Chronic pancreatitis (CP) is considered to result from repetitive pancreatic injury, and sustained production of various proinflammatory cytokines and chemokines are closely involved in its pathogenesis. Monocyte chemoattractant protein 1 (MCP-1), a member of the CC chemokine family, is believed to contribute to the progression of CP through monocyte/macrophage recruitment. This study aimed to clarify the protective role of thioredoxin-1 (TRX-1), a redox-regulating protein with antioxidative activity, in MCP-1 production and pancreatic fibrosis using a CP model in transgenic mice overexpressing TRX-1 (TRX-1-TG mice) and wildtype C57BL/6 mice. Experimental CP was induced by repeated administration of cerulein and lipopolysaccharide for 6 weeks. In TRX-1-TG mice, pancreatic atrophy was ameliorated, and histologically detectable inflammatory cell infiltration, glandular atrophy, and pseudotubular complex formation were suppressed. Overexpression of TRX-1 also attenuated pancreatic fibrosis and suppressed the activation of pancreatic stellate cells. Serum levels of MCP-1 and pancreatic expression of transforming growth factor-beta, platelet-derived growth factor, and MCP-1 were reduced in TRX-1-TG mice compared with levels in wild-type mice. Overexpression of TRX-1 also reduced H(2)O(2)-induced MCP-1 production in isolated pancreatic acinar cells. These results indicate that TRX-1 can potentially attenuate pancreatic fibrosis via the suppression of oxidative stress and MCP-1-mediated chronic inflammation.
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Authors | Shinya Ohashi, Akiyoshi Nishio, Hajime Nakamura, Masanori Asada, Hiroyuki Tamaki, Kimio Kawasaki, Toshiro Fukui, Junji Yodoi, Tsutomu Chiba |
Journal | Antioxidants & redox signaling
(Antioxid Redox Signal)
2006 Sep-Oct
Vol. 8
Issue 9-10
Pg. 1835-45
ISSN: 1523-0864 [Print] United States |
PMID | 16987036
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Actins
- Ccl2 protein, mouse
- Chemokine CCL2
- Lipopolysaccharides
- Platelet-Derived Growth Factor
- Proteins
- TXN protein, human
- Transforming Growth Factor beta
- Malondialdehyde
- Thioredoxins
- Ceruletide
- Hydrogen Peroxide
- Hydroxyproline
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Topics |
- Actins
(metabolism)
- Animals
- Atrophy
- Cells, Cultured
- Ceruletide
(toxicity)
- Chemokine CCL2
(blood, genetics)
- Disease Models, Animal
- Fibrosis
- Gene Expression
(genetics)
- Humans
- Hydrogen Peroxide
(pharmacology)
- Hydroxyproline
(metabolism)
- Lipopolysaccharides
(toxicity)
- Male
- Malondialdehyde
(analysis, metabolism)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Organ Size
- Pancreas
(drug effects, metabolism, pathology)
- Pancreas, Exocrine
(cytology, drug effects, metabolism)
- Pancreatitis, Chronic
(chemically induced, pathology, prevention & control)
- Platelet-Derived Growth Factor
(genetics)
- Proteins
(metabolism)
- Thioredoxins
(genetics, metabolism)
- Transforming Growth Factor beta
(genetics)
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