Airway responses in Brown Norway rats following inhalation sensitization and challenge with trimellitic anhydride.

Abstract

Trimellitic anhydride (TMA) is a cause of asthma in man. Dose-dependent TMA-specific IgE, histopathology, and airway responses after sensitization by inhalation were examined in the Brown Norway rat. Rats were exposed to 0.04, 0.4, 4, or 40 mg/m3 TMA aerosol for 10 min, once a week, over 10 weeks. All lower exposures were, subsequently, rechallenged to 40 mg/m3 TMA aerosol. All rats received a sham exposure 1 week prior to the first TMA exposure. Following the sham exposure and weekly after each TMA exposure, TMA-specific IgE and both early-phase airway response (EAR) and late-phase airway response (LAR) were measured using enhanced pause (Penh). All rats sensitized by 40 mg/m3 TMA developed specific IgE, EAR, and LAR to one or more of the challenges to 40 mg/m3 TMA. TMA of 4 mg/m3 induced a much lower, but stable, specific IgE response. EAR and LAR were observed only after a 40 mg/m3 TMA rechallenge in this group, but it was much larger than that observed in the 40 mg/m3 TMA-sensitized and challenged group. Exposure-dependent histopathological changes noted included eosinophilic granulomatous interstitial pneumonia, perivascular eosinophil infiltrates, bronchial-associated lymphoid tissue hyperplasia, and peribronchiolar plasma cell infiltrates.

• Authors: Xing-Dong Zhang, Michael E Andrew, Ann F Hubbs, Paul D Siegel
• Journal: Toxicological sciences : an official journal of the Society of Toxicology (Toxicol Sci) Vol. 94 Issue 2 Pg. 322-9 (Dec 2006) ISSN: 1096-6080 [Print] United States
• PMID: 16982671 (Publication Type: Journal Article)

Chemical References

• Allergens
• Antibodies, Anti-Idiotypic
• Phthalic Anhydrides
• Immunoglobulin E
• trimellitic anhydride

Topics

• Administration, Inhalation
• Airway Resistance (drug effects, physiology)
• Allergens (immunology, toxicity)
• Animals
• Antibodies, Anti-Idiotypic (blood)
• Bronchi (drug effects, pathology)
• Bronchial Hyperreactivity (chemically induced, immunology, pathology)
• Bronchial Provocation Tests
• Female
• Immunoglobulin E (blood, immunology)
• Inhalation Exposure
• Phthalic Anhydrides (immunology, toxicity)
• Plethysmography, Whole Body
• Rats
• Rats, Inbred BN
• Respiratory Hypersensitivity (chemically induced, immunology, pathology)
• Specific Pathogen-Free Organisms