Abstract | BACKGROUND: Although adverse health effects of particulate matter with a diameter of < 100 nm (nanoparticles) have been proposed, molecular and/or experimental evidence for their facilitation of lung inflammation in vivo is not fully defined. OBJECTIVE: RESULTS: We intratracheally administered vehicle, two sizes (14 nm, 56 nm) of carbon black nanoparticles (4 mg/kg) , LPS (2.5 mg/kg) , or LPS plus nanoparticles and evaluated parameters for lung inflammation and coagulation. Nanoparticles alone induced slight lung inflammation and significant pulmonary edema compared with vehicle. Fourteen-nanometer nanoparticles intensively aggravated LPS-elicited lung inflammation and pulmonary edema that was concomitant with the enhanced lung expression of interleukin-1beta (IL-1beta) , macrophage inflammatory protein-1alpha (MIP-1alpha) , macrophage chemoattractant protein-1, MIP-2, and keratinocyte chemoattractant in overall trend, whereas 56-nm nanoparticles did not show apparent effects. Immunoreactivity for 8-hydroxyguanosine, a marker for oxidative stress, was more intense in the lungs from the LPS + 14-nm nanoparticle group than in those from the LPS group. Circulatory fibrinogen levels were higher in the LPS + plus 14-nm nanoparticle group than in the LPS group. CONCLUSIONS: Taken together, evidence indicates that nanoparticles can aggravate lung inflammation related to bacterial endotoxin, which is more prominent with smaller particles. The enhancement may be mediated, at least partly, via the increased local expression of proinflammatory cytokines and via the oxidative stress. Furthermore, nanoparticles can promote coagulatory disturbance accompanied by lung inflammation.
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Authors | Ken-Ichiro Inoue, Hirohisa Takano, Rie Yanagisawa, Seishiro Hirano, Miho Sakurai, Akinori Shimada, Toshikazu Yoshikawa |
Journal | Environmental health perspectives
(Environ Health Perspect)
Vol. 114
Issue 9
Pg. 1325-30
(Sep 2006)
ISSN: 0091-6765 [Print] United States |
PMID | 16966083
(Publication Type: Journal Article)
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Chemical References |
- Air Pollutants
- Bacterial Toxins
- Ccr2 protein, mouse
- Chemokine CCL3
- Chemokine CCL4
- Chemokine CXCL2
- Chemokines
- Cxcl2 protein, mouse
- Interleukin-1beta
- Lipopolysaccharides
- Macrophage Inflammatory Proteins
- Receptors, CCR2
- Receptors, Chemokine
- Vehicle Emissions
- Fibrinogen
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Topics |
- Air Pollutants
(toxicity)
- Animals
- Bacterial Toxins
(toxicity)
- Chemokine CCL3
- Chemokine CCL4
- Chemokine CXCL2
- Chemokines
(metabolism)
- Fibrinogen
(metabolism)
- Interleukin-1beta
(metabolism)
- Keratinocytes
(metabolism)
- Lipopolysaccharides
(toxicity)
- Macrophage Inflammatory Proteins
(metabolism)
- Macrophages
(drug effects, metabolism)
- Male
- Mice
- Mice, Inbred ICR
- Nanoparticles
(toxicity)
- Pneumonia
(chemically induced, pathology)
- Pulmonary Edema
(chemically induced, pathology)
- Receptors, CCR2
- Receptors, Chemokine
(metabolism)
- Vehicle Emissions
(toxicity)
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