Abstract |
To investigate the expression and significance of X-linked inhibitor of apoptosis protein (XIAP) and XIAP-associated factor 1 (XAF1) in acute leukemia, the expression of XIAP, XAF1, Smac, and HtrA2 mRNA in the bone marrow aspirates from 87 newly diagnosed AL patients, 23 patients in remission, 6 patients in relapse, and 17 normal controls were detected by means of reverse transcriptase polymerase chain reaction (RT-PCR), and their relationship with clinical therapeutic efficiency was analyzed. The results showed that the expression level of XIAP mRNA in newly diagnosed AL patients (0.990 +/- 0.337) was significantly higher than that in normal controls (0.395 +/- 0.148) (P < 0.01); the positive rate and expression level of XAF1 mRNA in newly diagnosed AL patients (56.32%, 0.246 +/- 0.267) were significantly lower than that in normal controls (100%, 0.964 +/- 0.387) (P < 0.01). In 69 out of 87 newly diagnosed AL patients, efficacy remained evaluable. AL patients with high level of XIAP achieved a lower complete remission (CR) rate than patients with low level of XIAP (54.55% and 86.11%, respectively) (P < 0.01). XAF1 positive patients achieved a higher CR rate than XAF1 negative patients (86.84% and 51.61%, respectively) (P < 0.01). It is concluded that the overexpression of XIAP and negativity of XAF1 may be two adverse prognostic factors in AL patients.
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Authors | Guang-Hua Chen, Feng-Ru Lin, Jin-Hai Ren, Jing Chen, Jing-Nan Zhang, Yan Wang, Jing Wang |
Journal | Zhongguo shi yan xue ye xue za zhi
(Zhongguo Shi Yan Xue Ye Xue Za Zhi)
Vol. 14
Issue 4
Pg. 639-43
(Aug 2006)
ISSN: 1009-2137 [Print] China |
PMID | 16928290
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adaptor Proteins, Signal Transducing
- Apoptosis Regulatory Proteins
- DIABLO protein, human
- Inhibitor of Apoptosis Proteins
- Intracellular Signaling Peptides and Proteins
- Mitochondrial Proteins
- Neoplasm Proteins
- RNA, Messenger
- X-Linked Inhibitor of Apoptosis Protein
- XAF1 protein, human
- Serine Endopeptidases
- HTRA2 protein, human
- High-Temperature Requirement A Serine Peptidase 2
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Topics |
- Acute Disease
- Adaptor Proteins, Signal Transducing
- Apoptosis Regulatory Proteins
- High-Temperature Requirement A Serine Peptidase 2
- Humans
- Inhibitor of Apoptosis Proteins
(biosynthesis, genetics)
- Intracellular Signaling Peptides and Proteins
(genetics)
- Leukemia
(metabolism)
- Mitochondrial Proteins
(biosynthesis, genetics)
- Neoplasm Proteins
(biosynthesis, genetics)
- Prognosis
- RNA, Messenger
(biosynthesis, genetics)
- Serine Endopeptidases
(biosynthesis, genetics)
- X-Linked Inhibitor of Apoptosis Protein
(biosynthesis, genetics)
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