A transgenic zebrafish model of neutrophilic inflammation.

Abstract

We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced after transection of the tail of zebrafish larvae and that this inflammation subsequently resolves over a similar time course to mammalian systems. Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally induced inflammation can be inhibited by the addition of a pancaspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.

• Authors: Stephen A Renshaw, Catherine A Loynes, Daniel M I Trushell, Stone Elworthy, Philip W Ingham, Moira K B Whyte
• Journal: Blood (Blood) Vol. 108 Issue 13 Pg. 3976-8 (Dec 15 2006) ISSN: 0006-4971 [Print] United States
• PMID: 16926288 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Green Fluorescent Proteins
• Peroxidase

Topics

• Animals
• Animals, Genetically Modified
• Cell Count (methods)
• Disease Models, Animal
• Green Fluorescent Proteins
• Image Processing, Computer-Assisted
• Inflammation (genetics, immunology, pathology)
• Neutrophils (immunology, pathology)
• Peroxidase (genetics, immunology)
• Promoter Regions, Genetic
• Transgenes (immunology)
• Wounds and Injuries (genetics, immunology, pathology)
• Zebrafish (genetics, immunology)