Abstract |
Staining with peanut agglutinin (PNA) and with polyclonal antibody to S100 protein have both been recommended as aids in the diagnosis of histiocytosis X ( Langerhans' cell histiocytosis). Although a large body of literature attests to the utility of S100 staining in this condition, the few published studies that involve PNA staining have employed varying methods of radically different results. We studied formaldehyde solution-fixed, paraffin-embedded tissue from 29 cases of histiocytosis X by using standard avidin- biotin immunostaining for S100 protein, as well as two published methods of PNA staining. All cases stained positively for S100 protein. By utilizing prior trypsinization and a three-step procedure, all cases also stained with PNA. With a two-step PNA-staining procedure, however, 9 cases failed to stain with PNA, and 3 cases showed a diffuse staining pattern that was not considered characteristic of Langerhans' and histiocytosis X cells. We concluded that both the S100 and the three-step PNA procedures are sensitive methods for the diagnosis of histiocytosis X.
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Authors | M S Rabkin, C R Kjeldsberg, C T Wittwer, J Marty |
Journal | Archives of pathology & laboratory medicine
(Arch Pathol Lab Med)
Vol. 114
Issue 5
Pg. 511-5
(May 1990)
ISSN: 0003-9985 [Print] United States |
PMID | 1692202
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Lectins
- Peanut Agglutinin
- Plant Lectins
- S100 Proteins
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Topics |
- Adolescent
- Adult
- Arachis
- Child
- Child, Preschool
- Female
- Histiocytosis, Langerhans-Cell
(metabolism)
- Humans
- Immunologic Techniques
- Infant
- Langerhans Cells
(metabolism)
- Lectins
- Male
- Peanut Agglutinin
- Plant Lectins
- S100 Proteins
(metabolism)
- Staining and Labeling
(methods)
- Subcellular Fractions
(metabolism)
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