Abstract | BACKGROUND: METHODS: We investigated: (i) IL-13Ralpha1 expression by human lung mast cells (HLMC); (ii) the number of IL-13Ralpha1+ bronchial submucosal mast cells in subjects with asthma and normal controls and (iii) the effect of IL-13 priming on HLMC expression of high-affinity IgE receptor (FcepsilonRI), stem cell factor receptor (CD117), histamine release, proliferation, and survival. RESULTS: Human lung mast cell expressed IL-13Ralpha1 mRNA. IL-13Ralpha1 was highly expressed on the surface HLMC (82+/-9%). Bronchial submucosal mast cell IL-13Ralpha1 expression was higher in asthmatics (86+/-2%) than normal controls (78+/-2%; P=0.015). IL-13 priming for 30 min did not increase HLMC histamine release, in the presence or absence of SCF or in response to IgE/ anti-IgE activation. IL-13 priming for 5 days upregulated HLMC FcepsilonRI expression (22% increase in fluorescent intensity; P=0.003), increased histamine release following IgE/ anti-IgE activation by 56% (P=0.03) and increased proliferation by 50% (P=0.003) without affecting cell survival or CD117 expression. The IL-13 specific neutralizing antibody CAT-354 inhibited all IL-13 mediated effects. CONCLUSION: Human lung mast cell express IL-13Ralpha1 and activation by IL-13 for 5 days increased FcepsilonRI expression and proliferation. Histamine release was not affected by short-term priming with IL-13, but was upregulated by priming for 5 days suggesting that this effect was mediated by the increased FcepsilonRI expression. These data support the view that targeting IL-13 may be beneficial in the treatment of asthma.
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Authors | D Kaur, F Hollins, L Woodman, W Yang, P Monk, R May, P Bradding, C E Brightling |
Journal | Allergy
(Allergy)
Vol. 61
Issue 9
Pg. 1047-53
(Sep 2006)
ISSN: 0105-4538 [Print] Denmark |
PMID | 16918506
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-13
- Interleukin-13 Receptor alpha1 Subunit
- Receptors, IgE
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Topics |
- Cell Proliferation
- Cells, Cultured
- Humans
- Interleukin-13
(physiology)
- Interleukin-13 Receptor alpha1 Subunit
(biosynthesis, genetics)
- Lung
(cytology, immunology, metabolism)
- Mast Cells
(cytology, immunology, metabolism)
- Receptors, IgE
(biosynthesis, genetics)
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