Role of glyceryl trinitrate, a nitric oxide donor, in the renal ischemia-reperfusion injury of rats.

Abstract

BACKGROUND/AIMS:

Ischemia-reperfusion injury is a serious clinical situation which can cause serious morbidity and mortality. An experimental renal ischemia-reperfusion injury model was designed to evaluate the role of glyceryl trinitrate (GTN) on renal function and histology.

METHODS:

50 Wistar albino rats were used in our study. Five groups were formed: (1) sham-control group; (2) acute renal ischemia (ARI) group with placebo (0.9% NaCl) infusion; (3) GTN infusion with a 75 microg/kg/min dose prior to ARI was administered; (4) GTN infusion with a 150 microg/kg/min dose prior to ARI was given, and finally (5) 150 microg/kg/min GTN infusion after the ARI period was applied. Serum BUN and creatinine levels were measured for evaluation of renal function. T(max-sec), glomerular filtration rate (GFR), and T(max-min) results following a (99m)Tc-DTPA diuretic renal scintigraphy were used. Histological examination was performed on nephrectomy specimens.

RESULTS:

Groups 2 and 5 showed higher BUN, creatinine, and lower GFR values than the other groups (p = 0.0001). There was no difference in BUN, creatinine, and GFR levels between groups 2 and 5 (p = 0.971, p = 0.739, p = 0.393). Also the T(max-sec) values were higher in groups 2 and 5 compared with the other groups (p = 0.0001). The presence of tubular necrosis was different between groups and was higher in groups 2 and 5 (p = 0.002).

CONCLUSION:

The application of GTN, a nitric oxide donor, has caused significant improvement in renal function when applied prior to an experimentally designed renal ischemia-reperfusion model. But administration of GTN had no effect after occurrence of ischemia.

• Authors: Hasan Fehmi Kucuk, Levent Kaptanoglu, Figen Ozalp, Necmi Kurt, Sadik Bingul, Oguzhan Aziz Torlak, Elif Colak, Huseyin Akyol, Aylin Ege Gul
• Journal: European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes (Eur Surg Res) Vol. 38 Issue 5 Pg. 431-7 ( 2006) ISSN: 0014-312X [Print] Switzerland
• PMID: 16912481 (Publication Type: Journal Article)
• Copyright: Copyright (c) 2006 S. Karger AG, Basel.

Chemical References

• Nitric Oxide Donors
• Nitric Oxide
• Nitroglycerin
• Creatine

Topics

• Animals
• Blood Urea Nitrogen
• Creatine (blood)
• Glomerular Filtration Rate
• Kidney (pathology, physiopathology)
• Nitric Oxide (physiology)
• Nitric Oxide Donors
• Nitroglycerin
• Rats
• Rats, Wistar
• Reperfusion Injury (physiopathology)