Troglitazone and 9cis,11trans,13trans-conjugated linolenic acid: comparison of their antiproliferative and apoptosis-inducing effects on different colon cancer cell lines.

Abstract

BACKGROUND:

We have previously reported that troglitazone, a synthetic ligand for peroxisome proliferator-activated receptor gamma (PPARgamma), and bitter gourd seed oil rich in 9cis,11trans,13trans-conjugated linolenic acid (9c,11t,13t-CLN) prevent colon carcinogenesis. To evaluate the chemotherapeutic effect and potency of these compounds on colon cancer cells, we investigated their antiproliferative and apoptosis-inducing effects using different human colon cancer cell lines.

METHODS:

The antiproliferative and apoptosis-inducing effects of troglitazone and 9c,11t,13t-CLN were evaluated and compared using HT-29, DLD-1 and Caco-2 cells at different stages of enterocytic differentiation.

RESULTS:

Troglitazone and 9c,11t,13t-CLN decreased cell viability and induced apoptosis in three colon cancer cell lines. The susceptibility of HT-29, which expresses PPARgamma at high levels, to troglitazone and 9c,11t,13t-CLN was higher than that of Caco-2 cells with low levels of PPARgamma.

CONCLUSION:

Troglitazone and 9c,11t,13t-CLN exhibited more effective chemotherapeutic effects on HT-29 cells than on Caco-2 cells.

• Authors: Y Yasui, M Hosokawa, H Kohno, T Tanaka, K Miyashita
• Journal: Chemotherapy (Chemotherapy) Vol. 52 Issue 5 Pg. 220-5 ( 2006) ISSN: 0009-3157 [Print] Switzerland
• PMID: 16899971 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Chromans
• Linolenic Acids
• PPAR gamma
• RNA, Messenger
• Thiazolidinediones
• alpha-Linolenic Acid
• eleostearic acid
• Troglitazone

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Caco-2 Cells
• Cell Differentiation
• Cell Proliferation (drug effects)
• Chromans (pharmacology)
• Colonic Neoplasms (metabolism, pathology)
• HT29 Cells
• Humans
• Linolenic Acids (pharmacology)
• PPAR gamma (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Thiazolidinediones (pharmacology)
• Troglitazone
• alpha-Linolenic Acid