Abstract |
Nonsteroidal anti-inflammatory drugs ( NSAIDs) have demonstrated cancer chemoprevention effects associated with their ability to modulate polyunsaturated fatty acid metabolism. In the present study, we report a significant reduction of 15-lipoxygenase 2 (15-LOX-2) in seven carcinoma cell lines of the human head and neck when compared with normal primary cultured keratinocytes, and 18 primary head and neck squamous cell carcinomas ( HNSCC) when compared with matched normal mucosa. 15-LOX-2 is mainly expressed in the mature cells of the benign squamous epithelium, but not in the basal layer cells of benign epithelium, suggesting a role of 15-LOX-2 in cell differentiation. We further found that 15-lipoxygenase activity was reduced in carcinoma cells when compared with normal primary cultured keratinocytes. When the effects of NSAIDs were examined on cell proliferation and regulation of 15-LOX-2 in the carcinoma cells, NS398 treatment resulted in significant growth inhibition associated with upregulation of 15-LOX-2 and its major metabolite 15-S-HETE. Finally, restoration of 15-LOX-2 expression into these carcinoma cells significantly inhibited cell proliferation. Our results demonstrate that 15-LOX-2 expression is significantly reduced and this reduction may promote proliferation in human head and neck carcinoma. 15-LOX-2 may be a possible biomarker in human head and neck malignancy.
|
Authors | Dian Wang, Shaoying Chen, Ying Feng, Qiuhui Yang, Bruce H Campbell, Xin Tang, William B Campbell |
Journal | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
(Tumour Biol)
Vol. 27
Issue 5
Pg. 261-73
( 2006)
ISSN: 1010-4283 [Print] Netherlands |
PMID | 16874012
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright (c) 2006 S. Karger AG, Basel. |
Chemical References |
- Nitrobenzenes
- Sulfonamides
- N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
- ALOX15B protein, human
- Arachidonate 15-Lipoxygenase
|
Topics |
- Arachidonate 15-Lipoxygenase
(metabolism)
- Carcinoma
(enzymology)
- Carcinoma, Squamous Cell
- Cell Proliferation
- Cells, Cultured
- Gene Expression Regulation, Enzymologic
- Head and Neck Neoplasms
(enzymology)
- Humans
- Keratinocytes
(enzymology)
- Nitrobenzenes
(pharmacology)
- Sulfonamides
(pharmacology)
- Tumor Cells, Cultured
|