Abstract | BACKGROUND:
Carbon dioxide (CO2) pneumoperitoneum has been shown to attenuate the inflammatory response after laparoscopy. This study tested the hypothesis that abdominal insufflation with CO2 improves survival in an animal model of sepsis and investigated the associated mechanism. METHODS: RESULTS: CONCLUSION: Abdominal insufflation with CO2, but not helium or air, significantly reduces mortality among animals with LPS-induced sepsis. Furthermore, CO2 pneumoperitoneum rescues animals from abdominal sepsis after a laparotomy. Because IL-10 is known to downregulate TNF-alpha, the increase in IL-10 and the decrease in TNF-alpha found among the CO2-insufflated animals in our study provide evidence for a mechanism whereby CO2 pneumoperitoneum reduces mortality via IL-10-mediated downregulation of TNF-alpha.
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Authors | E J Hanly, J M Fuentes, A R Aurora, S L Bachman, A De Maio, M R Marohn, M A Talamini |
Journal | Surgical endoscopy
(Surg Endosc)
Vol. 20
Issue 9
Pg. 1482-7
(Sep 2006)
ISSN: 1432-2218 [Electronic] Germany |
PMID | 16865628
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Lipopolysaccharides
- Tumor Necrosis Factor-alpha
- Interleukin-10
- Carbon Dioxide
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Topics |
- Abdomen
(microbiology)
- Animals
- Carbon Dioxide
- Down-Regulation
- Interleukin-10
(metabolism)
- Laparotomy
(adverse effects)
- Lipopolysaccharides
- Male
- Pneumoperitoneum, Artificial
- Rats
- Rats, Sprague-Dawley
- Salvage Therapy
- Sepsis
(chemically induced, etiology, mortality)
- Survival Analysis
- Tumor Necrosis Factor-alpha
(antagonists & inhibitors)
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