Abstract |
We evaluated the cytotoxic and apoptotic effects of two purine nucleoside analogues, acyclovir (ACV) and ganciclovir (GCV), on lymphoma cells stably harboring Kaposi's sarcoma-associated herpesvirus (KSHV). Colorimetric caspase assay, flow cytometry, and immunoblotting with antibodies against apoptosis-related molecules revealed that GCV has cytotoxic activity toward KSHV-infected primary effusion lymphoma cells, while ACV has weak or little activity. In addition to the GCV-induced cytotoxicity, apoptosis via caspase-7/8, cleavage of poly(ADP-ribose) polymerase, and accumulation of p53 and p21 were induced by GCV treatment. In contrast, neither ACV nor GCV have cytotoxicity- or apoptosis-inducing activities toward uninfected cells.
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Authors | Masahiro Fujimuro, Hisanori Inoue, Yasuhiro Teishikata, Yuriko Ishida, Hideyoshi Yokosawa |
Journal | Nucleosides, nucleotides & nucleic acids
(Nucleosides Nucleotides Nucleic Acids)
Vol. 25
Issue 4-6
Pg. 635-45
( 2006)
ISSN: 1525-7770 [Print] United States |
PMID | 16838851
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Caspase 3
- Caspase 7
- Ganciclovir
- Acyclovir
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Topics |
- Acyclovir
(pharmacology)
- Apoptosis
(drug effects)
- Caspase 3
(metabolism)
- Caspase 7
(metabolism)
- Ganciclovir
(pharmacology, therapeutic use)
- Herpesvirus 8, Human
(physiology)
- Humans
- Lymphoma
(drug therapy, pathology, virology)
- Tumor Cells, Cultured
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