Release of potential immunomodulatory factors during platelet storage.

Abstract

BACKGROUND:

Blood platelets (PLTs) link the processes of hemostasis and inflammation. Recent studies have demonstrated that PLTs promote immunity and inflammation mainly by means of the CD40/CD40L pathway. Our objective was to describe the accumulation of cytokines in PLT concentrates during storage.

STUDY DESIGN AND METHODS:

Pools of PLT concentrates were prepared, separated from plasma, and resuspended in clinical-grade storage medium; samples were taken on Days 0, 1, 2, 3, and 5 for analysis, without replacement (i.e., without soluble protein dilution). Interleukin (IL)-6, IL-8, PLT-derived growth factor (PDGF)-AA, soluble CD40 ligand (sCD40L), RANTES, and transforming growth factor-beta production were measured by specific enzyme-linked immunosorbent assays.

RESULTS:

Over time, the levels of RANTES, IL-8, and IL-6 were stable. In contrast, the levels of PDGF-AA and sCD40L increased. Ex vivo production of sCD40L was quantified at levels sufficient to induce B-cell effects based on previous studies of in vitro induced B-cell activation and differentiation by sCD40L. Cytokine and/or chemokine levels were generally higher in PLT concentrate supernatants and/or PLT lysates in comparison to PLT-free plasma, allowing the determination of which cytokine and/or chemokine was absorbed or secreted by transfusion-grade PLTs over time.

CONCLUSION:

Our data provide evidence that stored PLTs contain molecules with known immunomodulatory competence and secrete them differentially over time during storage for transfusion purposes.

• Authors: Fabrice Cognasse, Françoise Boussoulade, Patricia Chavarin, Sophie Acquart, Patrick Fabrigli, Bernard Lamy, Olivier Garraud
• Journal: Transfusion (Transfusion) Vol. 46 Issue 7 Pg. 1184-9 (Jul 2006) ISSN: 0041-1132 [Print] United States
• PMID: 16836566 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokine CCL5
• Cytokines
• Immunologic Factors
• Interleukins
• Platelet-Derived Growth Factor
• platelet-derived growth factor A
• CD40 Ligand

Topics

• Blood Platelets (immunology, metabolism)
• Blood Preservation
• CD40 Ligand (analysis)
• Chemokine CCL5 (analysis)
• Cytapheresis
• Cytokines (analysis)
• Humans
• Immunologic Factors (metabolism)
• Interleukins (analysis)
• Platelet Transfusion (methods, standards)
• Platelet-Derived Growth Factor (analysis)
• Time Factors