[Dipyridamole preconditioning protects against ischemia/reperfusion injury of rat liver].

Abstract

OBJECTIVE:

To investigate the protective mechanism of dipyridamole preconditioning against hepatic ischemia/reperfusion injury.

METHODS:

Thirty SD rats were randomly divided into normal control group, ischemia/reperfusion group, and dipyridamole group, with 10 rats in each group. Using 45-minute ischemia/reperfusion rat model at normal temperature, 10 mg/kg of dipyridamole normal saline were injected into portal vein before ischemia. Only normal saline was injected in the ischemia/reperfusion group. An hour later, blood was obtained from the portal vein to determine the enzyme levels, including alanine aminotransferase (ALT) , lactate dehydrogenase (LDH) and tumor necrosis factor-alpha (TNF-alpha), and endothelin-1 (ET-1). The alteration in pathological morphology of the ischemia lobe was observed. The content of adenosine phosphates in the liver was determined.

RESULTS:

The ALT and LDH activity, TNF-alpha, ET-1 levels were significantly higher in the ischemia/reperfusion group than those in the control group (all P<0.01). The levels of these variables were much lower in the dipyridamole group than those of the ischemia/reperfusion group (P<0.01), but little higher than those of the control group (P>0.05). The adenosine phosphates levels of ischemia/reperfusion group were significantly lower than those of the control group (P<0.01). They were much higher in the dipyridamole group than those of the ischemia/reperfusion group (P<0.01), but little higher than those of the control group (P>0.05). The control group had obvious alteration in pathological morphology, but only slight alteration was found in dipyridamole group, compared with control group.

CONCLUSION:

Dipyridamole preconditioning protects against ischemia/reperfusion injury of the liver.

• Authors: Yong Hong, Wen-sheng Liao, Rui-xiang Mo
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 18 Issue 7 Pg. 425-7 (Jul 2006) ISSN: 1003-0603 [Print] China
• PMID: 16831247 (Publication Type: English Abstract, Journal Article)

Chemical References

• Dipyridamole

Topics

• Animals
• Dipyridamole (therapeutic use)
• Disease Models, Animal
• Female
• Ischemic Preconditioning
• Liver (blood supply, metabolism, pathology)
• Male
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology, prevention & control)