Abstract | BACKGROUND: MATERIALS AND METHODS: MCF-7 human breast cancer cells were exposed to vehicle alone, 10 microM MTX, 1 microM 5-FU, 10 microM RAL, 10 microM RAL 24 hours prior to 1 microM 5-FU followed 2 hours later by 10 microM MTX, and 1 microM 5-FU 2 hours prior to 10 microM MTX followed 24 hours later by 10 microM RAL. The cells were evaluated for viability and proliferation. The retinoblastoma ( Rb) protein, a cell cycle regulator which when phosphorylated allows the progression of cells from G1- to S-phase, was used as a marker to determine the effects of early RAL and late RAL on cellular progression at the molecular level. RESULTS: Early RAL administration exhibited a cell viability of 66.83 +/- 6.17% of the control. However, late RAL administration exhibited cell viability 39.40 +/- 17.03% of the control. Late RAL was a more cytotoxic combination than RAL alone or early RAL. These findings from manual cell counting were also supported by cell flow cytometric analysis and Western blot data. CONCLUSION: Late RAL in combination with 5-FU and MTX, due to greater cytotoxicity, is a more desirable combination to treat breast cancer than RAL alone.
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Authors | Elizabeth B Fryar, Jharna R Das, Jillian H Davis, Joseph A Desoto, Ibrahim Laniyan, William M Southerland, Donnell Bowen |
Journal | Anticancer research
(Anticancer Res)
2006 May-Jun
Vol. 26
Issue 3A
Pg. 1861-7
ISSN: 0250-7005 [Print] Greece |
PMID | 16827118
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Raloxifene Hydrochloride
- Fluorouracil
- Methotrexate
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Topics |
- Antineoplastic Combined Chemotherapy Protocols
(administration & dosage, adverse effects)
- Blotting, Western
- Breast Neoplasms
(drug therapy, pathology)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Drug Administration Schedule
- Drug Interactions
- Flow Cytometry
- Fluorouracil
(administration & dosage, adverse effects)
- Humans
- Methotrexate
(administration & dosage, adverse effects)
- Raloxifene Hydrochloride
(administration & dosage)
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