Abstract | BACKGROUND: METHODS AND RESULTS: Wistar rats weighing 200 grams were infected intraperitoneally with 15 x 10(4) trypomastigotes. After 8 months, 2-dimensional echocardiographic study was performed for baseline assessment of left ventricle (LV) ejection fraction (EF) (%), left ventricle end-diastolic volume (LVEDV) (mL), and left ventricle end-systolic volume (LVESV) (mL). Animals with LV dysfunction (EF <37%) were selected for the study. Autologous skeletal myoblasts were isolated from muscle biopsy and mesenchymal stem cells from bone marrow aspirates were co-cultured in vitro for 14 days, yielding a cell viability of >90%. Eleven animals received autologous transplant of 5.4 x 10(6)+/-8.0 x 10(6) cells (300 microL) into the LV wall. The control group (n=10) received culture medium (300 microL). Cell types were identified with vimentin and fast myosin. After 4 weeks, ventricular function was reassessed by echo. For histological analysis, heart tissue was stained with hematoxylin and eosin and immunostained for fast myosin. After 4 weeks, cell transplantation significantly improved EF and reduced LVEDV and LVESV. No change was observed in the control group. CONCLUSIONS:
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Authors | L C Guarita-Souza, K A T Carvalho, V Woitowicz, C Rebelatto, A Senegaglia, P Hansen, N Miyague, J C Francisco, M Olandoski, J R Faria-Neto, P Brofman |
Journal | Circulation
(Circulation)
Vol. 114
Issue 1 Suppl
Pg. I120-4
(Jul 04 2006)
ISSN: 1524-4539 [Electronic] United States |
PMID | 16820560
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Cardiomyopathy, Dilated
(diagnostic imaging, etiology, physiopathology, surgery)
- Cells, Cultured
(transplantation)
- Chagas Cardiomyopathy
(diagnostic imaging, physiopathology, surgery)
- Coculture Techniques
- Coronary Circulation
- Fibrosis
- Mesenchymal Stem Cell Transplantation
- Mesenchymal Stem Cells
(cytology)
- Microcirculation
- Muscle, Skeletal
(cytology)
- Myoblasts
(cytology, transplantation)
- Myocardium
(pathology)
- Rats
- Rats, Wistar
- Stroke Volume
- Ultrasonography
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