The intradermal Leishmanin reaction induces antigen-specific maturation of canine dendritic cells with up-regulation of MHCII synthesis and expression.

Abstract

Dendritic cells (DCs) are professional antigen-presenting cells that reside in many tissues, including the skin. This study showed that intradermal injection of leishmanin in Leishmania infantum-infected dogs induced the "up-regulation" of surface MHCII expression, associated with progressive ultrastrucutural changes characteristic of DC maturation, including the formation of multilaminar MHC class II-containing compartments and arrays of tubulo-vesicular structures. These changes were not observed in control dogs from L. infantum non-endemic areas. The results indicated that canine DCs were effector cells in delayed-type hypersensitivity, that the leishmanin reaction was specific for a cell-mediated reaction to L. infantum in infected dogs, and that canine DCs possessed ultrastructural organelles reminiscent of those in activated human DCs.

• Authors: L Sacchi, L E Calvi, L H Kramer, E Ferroglio, G Grandi, E Clementi, S Corona
• Journal: Journal of comparative pathology (J Comp Pathol) Vol. 135 Issue 1 Pg. 17-24 (Jul 2006) ISSN: 0021-9975 [Print] England
• PMID: 16814802 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antigens
• Antigens, Protozoan
• Histocompatibility Antigens Class II
• leishmanin

Topics

• Animals
• Antigens (immunology, metabolism)
• Antigens, Protozoan (immunology)
• Dendritic Cells (cytology, immunology, metabolism, parasitology, ultrastructure)
• Dogs
• Histocompatibility Antigens Class II (biosynthesis, physiology, ultrastructure)
• Leishmania infantum (immunology)
• Up-Regulation