Abstract | BACKGROUND: METHODS: The expression of several genes related to the adenosinergic system (ADORA1A, ADORA2A, ADORA2B) were studied in the brain of the murine model of LND by real time PCR. Nucleotide levels and enzyme activities possibly involved in adenosine release were also measured. RESULTS: Studies performed by real time PCR showed 95% increase in ADORA1A expression, 15% decrease in ADORA2A expression, and no change in ADORA2B expression in knockout mice compared to controls. No significant differences were found in the level of nucleotides or enzyme activities between control and mutant mice. CONCLUSIONS: Our results suggest that adenosine neurotransmission might be involved in the specific neurobehavioral features of LND by increased expression of adenosine A1 receptors.
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Authors | Matteo Bertelli, Stefano Cecchin, Cristina Lapucci, Gabriella Jacomelli, Hyder A Jinnah, Massimo Pandolfo, Vanna Micheli |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 373
Issue 1-2
Pg. 104-7
(Nov 2006)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 16793031
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Receptor, Adenosine A1
- Receptors, Purinergic P1
- Hypoxanthine Phosphoribosyltransferase
- Adenosine
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Topics |
- Adenosine
(metabolism)
- Animals
- Brain
(metabolism, pathology)
- Disease Models, Animal
- Gene Expression Profiling
- Hypoxanthine Phosphoribosyltransferase
(deficiency, metabolism)
- Lesch-Nyhan Syndrome
(genetics, metabolism, pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- RNA, Messenger
(biosynthesis)
- Receptor, Adenosine A1
(genetics)
- Receptors, Purinergic P1
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
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